A0A2B7WKJ6 · A0A2B7WKJ6_POLH7
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1197 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 162 | ATP (UniProtKB | ChEBI) | |||
Binding site | 246 | ATP (UniProtKB | ChEBI) | |||
Binding site | 281 | ATP (UniProtKB | ChEBI) | |||
Active site | 338 | ||||
Binding site | 591 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 663 | substrate | |||
Binding site | 759 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 789 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 791 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 924 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Onygenales > Onygenales incertae sedis > Polytolypa
Accessions
- Primary accessionA0A2B7WKJ6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 759 | N6-carboxylysine | |||
Modified residue | 1160 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 44-496 | Biotin carboxylation | |||
Domain | 166-363 | ATP-grasp | |||
Domain | 582-850 | Pyruvate carboxyltransferase | |||
Domain | 1119-1194 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,197
- Mass (Da)131,248
- Last updated2017-12-20 v1
- ChecksumE72FDDC3ECBC0BE1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
PDNA01000335 EMBL· GenBank· DDBJ | PGG97068.1 EMBL· GenBank· DDBJ | Genomic DNA |