A0A2A8DCT4 · A0A2A8DCT4_9LACT
- ProteinD-aminoacyl-tRNA deacylase
- Genedtd
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids151 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.
Catalytic activity
- a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H+
- glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H+
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | D-tyrosyl-tRNA(Tyr) deacylase activity | |
Molecular Function | Gly-tRNA(Ala) hydrolase activity | |
Molecular Function | Ser(Gly)-tRNA(Ala) hydrolase activity | |
Molecular Function | tRNA binding | |
Biological Process | D-amino acid catabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-aminoacyl-tRNA deacylase
- EC number
- Short namesDTD
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Lactococcus
Accessions
- Primary accessionA0A2A8DCT4
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 136-137 | Gly-cisPro motif, important for rejection of L-amino acids | |||
Domain
A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.
Sequence similarities
Belongs to the DTD family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length151
- Mass (Da)16,532
- Last updated2017-12-20 v1
- Checksum1318BD19AC88A52B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JAOWLP010000008 EMBL· GenBank· DDBJ | MDG4981920.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAOWLY010000001 EMBL· GenBank· DDBJ | MDG4982933.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
NCWV01000005 EMBL· GenBank· DDBJ | PAK89267.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RAGV01000007 EMBL· GenBank· DDBJ | RQE32536.1 EMBL· GenBank· DDBJ | Genomic DNA |