A0A2A4NS30 · A0A2A4NS30_9FLAO
- ProteinNitrate reductase
- GenenapA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids774 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalytic subunit of the nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.
Catalytic activity
- 2 Fe(II)-[cytochrome] + nitrate + 2 H+ = 2 Fe(III)-[cytochrome] + nitrite + H2O
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
Note: Binds 1 [4Fe-4S] cluster.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 53 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 56 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 60 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 88 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 90 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 150 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 175 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 179 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 261-263 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 363 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 367 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 472 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 497-498 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 520 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 547 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 664-673 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 740 | substrate | |||
Binding site | 748 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
Binding site | 765 | Mo-bis(molybdopterin guanine dinucleotide) (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | electron transfer activity | |
Molecular Function | iron ion binding | |
Molecular Function | molybdenum ion binding | |
Molecular Function | molybdopterin cofactor binding | |
Molecular Function | nitrate reductase (cytochrome) activity | |
Biological Process | Mo-molybdopterin cofactor biosynthetic process | |
Biological Process | nitrate assimilation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitrate reductase
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageBacteria > Bacteroidota > Flavobacteriia > Flavobacteriales > Flavobacteriaceae
Accessions
- Primary accessionA0A2A4NS30
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Membrane-associated.
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-34 | ||||
Chain | PRO_5012314140 | 35-774 | Nitrate reductase | ||
Post-translational modification
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Interaction
Subunit
Component of the nitrate reductase NapAB complex composed of NapA and NapB.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 46-102 | 4Fe-4S Mo/W bis-MGD-type | |||
Sequence similarities
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length774
- Mass (Da)86,939
- Last updated2017-12-20 v1
- MD5 Checksum76C8D005BC9918B508EF07876F67EBF6
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
NVRM01000088 EMBL· GenBank· DDBJ | PCH75259.1 EMBL· GenBank· DDBJ | Genomic DNA |