A0A291MVS8 · A0A291MVS8_SPHYA

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site49
Binding site52ATP (UniProtKB | ChEBI)
Binding site91ATP (UniProtKB | ChEBI)
Binding site93Mg2+ 1 (UniProtKB | ChEBI)
Binding site94-97substrate
Active site95Proton acceptor
Binding site116substrate
Binding site117Mg2+ 2 (UniProtKB | ChEBI)
Binding site240substrate
Binding site268Mg2+ 2 (UniProtKB | ChEBI)
Binding site312-314substrate
Binding site497ATP (UniProtKB | ChEBI)
Binding site534ATP (UniProtKB | ChEBI)
Binding site535Mg2+ 1 (UniProtKB | ChEBI)
Binding site537substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL
    • ORF names
      A6768_02675

Organism names

Accessions

  • Primary accession
    A0A291MVS8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain15-53Phosphoribosylformylglycinamidine synthase linker
Domain74-189PurM-like N-terminal
Domain202-356PurM-like C-terminal
Domain440-562PurM-like N-terminal
Domain577-673PurM-like C-terminal

Sequence similarities

Belongs to the FGAMS family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    725
  • Mass (Da)
    76,374
  • Last updated
    2017-12-20 v1
  • Checksum
    3D95145FFE136A9E
MSSSAPQITPQIVAEHGLSPEEYDRVLKAIGREPNLTELGIFSVMWSEHCSYKSSKIHLMKLPTKGPQVICGPGENAGVVDIGDGQAAIFKMESHNHPSYIEPYQGAATGVGGILRDVFTMGARPVANMNALRFGRPDHPKMKHLISGVVHGIGGYGNCVGVPTVGGEVNFHPAYDGNILVNAMTVGIADTDKIFYSAASGVGNPIVYVGSKTGRDGIHGATMASADFGEDSEEKRPTVQVGDPFTEKLLIEACLELMASDAIVAIQDMGAAGLTSSSVEMASKGGVGLHLKMDDVPQRETGMTTYEMMLSESQERMLMVLKPGREAFAEAIFRKWELDFAVIGHVTDTGRMVLEHKGEIVCDIPLAPLADDAPLYDRPSMPKDEYKIWSKVEPLGEIAESANIGADLVTLMGSPDIASRRWIWEQYDHMVGADTVQRPGGDAAVVRVHGSSKGIAISTDCTPRYCYADPYEGGKQAIAECYRNLSAVGSTPLAVTNCLNFANPQRPEIMAQLTGCLEGMGDACRAFDFPIVSGNVSLYNESKATGGGSAILPTPAIGGIGLLADIDAMATVAFKNEGDAIWLVGGEGTHLGQSIWLREIAGREAGDAPKVDLAVERANGDAVRALVVAGTVNAVHDISDGGLIVTIAEMALAGKIGATLDVALTTASAFGEDQSRYVVTAPAGVEIPGAVKIGTVGGDSVADVSIDALRTAHEGFFPALMDSEL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP023741
EMBL· GenBank· DDBJ
ATI79018.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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