A0A291B633 · A0A291B633_9GAMC

  • Protein
    ORF1ab polyprotein
  • Gene
    1ab
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2 (By similarity).
Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses
Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities (By similarity).
Therefore plays an essential role in viral mRNAs cap methylation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site5555
Active site5557
Active site5656
Active site5732
Active site5737
Binding site5795-5801S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6210
Active site6225
Active site6265

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Gene names

    • Name
      1ab

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 1148-A
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Gammacoronavirus > Igacovirus

Accessions

  • Primary accession
    A0A291B633

Subcellular Location

Cytoplasm
Endoplasmic reticulum membrane
; Multi-pass membrane protein
Host endoplasmic reticulum membrane
; Multi-pass membrane protein
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1744-1760Helical
Transmembrane1841-1868Helical
Transmembrane1880-1905Helical
Transmembrane2278-2296Helical
Transmembrane2555-2577Helical
Transmembrane2583-2604Helical
Transmembrane2611-2628Helical
Transmembrane2634-2662Helical
Transmembrane3094-3114Helical
Transmembrane3120-3137Helical
Transmembrane3149-3168Helical
Transmembrane3188-3206Helical
Transmembrane3218-3236Helical
Transmembrane3256-3279Helical
Transmembrane3291-3318Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1799↔1805

Keywords

Interaction

Subunit

Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure (By similarity).
Interacts with ORF6 protein
Homodimer.
Interacts with host PHB and PHB2.
Interacts with papain-like protease and non-structural protein 6.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain675-780Ubiquitin-like
Domain1002-1178Macro
Domain1174-1226Ubiquitin-like
Domain1235-1494Peptidase C16
Domain1766-18303Ecto
Region1908-1998Y1
Domain1908-2260CoV Nsp3 Y
Region1912-1925ZF1
Region1958-1968ZF2
Region1999-2260CoV-Y
Region2161-2260Y4
Domain2681-2776Nsp4C
Domain2777-3083Peptidase C30
Domain3378-3460RdRp Nsp7 cofactor
Domain3461-3670RdRp Nsp8 cofactor
Domain3671-3781Nsp9 ssRNA-binding
Domain3783-3924ExoN/MTase coactivator
Domain3938-4196NiRAN
Domain4201-4299Nsp12 Interface
Domain4300-4866Nsp12 RNA-dependent RNA polymerase
Domain4546-4708RdRp catalytic
Domain4867-4950CV ZBD
Domain5123-5475+RNA virus helicase C-terminal
Domain5537-5751ExoN
Domain5760-5987N7-MTase
Region5875-5889GpppA-binding
Domain5988-6048Nsp15 N-terminal oligomerization
Domain6049-6164AV-Nsp11N/CoV-Nsp15M
Domain6181-6322NendoU
Domain6325-6624Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,627
  • Mass (Da)
    743,839
  • Last updated
    2017-12-20 v1
  • Checksum
    BFA9A029D8BEFD6C

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KY933089
EMBL· GenBank· DDBJ
ATE90965.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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