A0A286YDB8 · A0A286YDB8_MOUSE
- ProteinElongator complex protein 3
- GeneElp3
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids553 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score2/5
Function
function
Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine34 acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.
Catalytic activity
- uridine34 in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine34 in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H+This reaction proceeds in the forward direction.
RHEA-COMP:11727 CHEBI:65315 Position: 34+ CHEBI:57288 + CHEBI:59789 + CHEBI:15377 = RHEA-COMP:10407 CHEBI:74882 Position: 34+ CHEBI:17319 + CHEBI:57844 + CHEBI:57287 + 2 CHEBI:15378
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
tRNA modification.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 99 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 109 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 112 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | iron-sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA uridine(34) acetyltransferase activity | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameElongator complex protein 3
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionA0A286YDB8
Proteomes
Organism-specific databases
Expression
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length553
- Mass (Da)63,054
- Last updated2017-11-22 v1
- MD5 ChecksumC4ED2ABBF942D18F341E16284ADEFB4E
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q9CZX0 | ELP3_MOUSE | Elp3 | 547 |
Keywords
- Technical term