A0A286LEZ6 · PSIK_PSICY

  • Protein
    4-hydroxytryptamine kinase
  • Gene
    psiK
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

4-hydroxytryptamine kinase; part of the gene cluster that mediates the biosynthesis of psilocybin, a psychotropic tryptamine-derived natural product (PubMed:28763571, PubMed:30283667).
The first step in the pathway is the decarboxylation of L-tryptophan to tryptamine by the decarboxylase psiD. PsiD does not decarboxylate phenylalanine, tyrosine, or 5-hydroxy- L -tryptophan (5-HTP) (PubMed:30283667).
4-hydroxy-L-tryptophan is accepted as substrate by psiD as well. The cytochrome P450 monooxygenase psiH then converts tryptamine to 4-hydroxytryptamine. The kinase psiK catalyzes the 4-O-phosphorylation step by converting 4-hydroxytryptamine into norbaeocystin. The methyltransferase psiM then catalyzes iterative methyl transfer to the amino group of norbaeocystin to yield psilocybin via a monomethylated intermediate, baeocystin. 4-hydroxy-6-methyl-l-tryptophancan also be converted the decarboxylase PsiD, kinase PsiK, and methyltransferase PsiM into respectively 6-methyl-norbaeocystin, 6-methylbaeocystin, and 6-methylpsilocybin (By similarity).
PsiK kinase can also turn psilocin into psilocybin (PubMed:35583969).
This activity may represent a protective mechanism to rephosphorylate the unstable psilocin to the stable psilocybin in case of intracellular ester cleavage (PubMed:35583969).
Moreover, psiK is able to O-phosphorylate the quaternary amine 4-hydroxy-N,N,N-trimethyltryptamine (4-OH-TMT) to yield aeruginascin, another bioactive compound found in Psilocybe species (PubMed:35583969).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Biotechnology

The pharmaceutical interesting psilocybin as a treatment option against depression and anxiety is being investigated in advanced clinical trials.

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site37ATP (UniProtKB | ChEBI)
Binding site57ATP (UniProtKB | ChEBI)
Binding site118-120ATP (UniProtKB | ChEBI)
Active site224
Binding site248-250ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4-hydroxytryptamine kinase activity
Molecular FunctionATP binding
Biological Processpsilocybin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxytryptamine kinase
  • EC number
  • Alternative names
    • Psilocybin biosynthesis kinase

Gene names

    • Name
      psiK

Organism names

  • Taxonomic identifier
  • Strain
    • FSU 12416
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Agaricomycetidae > Agaricales > Agaricineae > Strophariaceae > Psilocybe

Accessions

  • Primary accession
    A0A286LEZ6

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004458281-3614-hydroxytryptamine kinase

Interaction

Subunit

Monomer.

Family & Domains

Sequence similarities

Belongs to the methylthioribose kinase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    361
  • Mass (Da)
    40,315
  • Last updated
    2017-11-22 v1
  • Checksum
    F3AD5838DA19F35A
MTFDLKTEEGLLSYLTKHLSLDVAPNGVKRLSGGFVNVTWRVGLNAPYHGHTSIILKHAQPHLSSDIDFKIGVERSAYEYQALKIVSANSSLLGSSDIRVSVPEGLHYDVVNNALIMQDVGTMKTLLDYVTAKPPISAEIASLVGSQIGAFIARLHNLGRENKDKDDFKFFSGNIVGRTTADQLYQTIIPNAAKYGIDDPILPIVVKELVEEVMNSEETLIMADLWSGNILLQFDENSTELTRIWLVDWELCKYGPPSLDMGYFLGDCFLVARFQDQLVGTSMRQAYLKSYARNVKEPINYAKATAGIGAHLVMWTDFMKWGNDEEREEFVKKGVEAFHEANEDNRNGEITSILVKEASRT

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KY984102
EMBL· GenBank· DDBJ
ASU62240.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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