A0A263NXJ3 · A0A263NXJ3_9PSED
- ProteinDihydroorotate dehydrogenase (quinone)
- GenepyrD
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids339 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
Catalytic activity
- (S)-dihydroorotate + a quinone = a quinol + orotate
Cofactor
Note: Binds 1 FMN per subunit.
Pathway
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 61-65 | FMN (UniProtKB | ChEBI) | ||||
Sequence: AGLDK | ||||||
Binding site | 65 | substrate | ||||
Sequence: K | ||||||
Binding site | 85 | FMN (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 110-114 | substrate | ||||
Sequence: NRMGF | ||||||
Binding site | 138 | FMN (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 171 | FMN (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 171 | substrate | ||||
Sequence: N | ||||||
Active site | 174 | Nucleophile | ||||
Sequence: S | ||||||
Binding site | 176 | substrate | ||||
Sequence: N | ||||||
Binding site | 216 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 244 | FMN (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 245-246 | substrate | ||||
Sequence: NT | ||||||
Binding site | 267 | FMN (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 296 | FMN (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 317-318 | FMN (UniProtKB | ChEBI) | ||||
Sequence: YT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | dihydroorotate dehydrogenase (quinone) activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydroorotate dehydrogenase (quinone)
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionA0A263NXJ3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 46-333 | Dihydroorotate dehydrogenase catalytic | ||||
Sequence: VSVMGLDFPNPVGLAAGLDKNGAAIDGFAQLGFGFVEIGTVTPRPQPGNPKPRIFRLPEAEAIINRMGFNNLGVDHLLSRVHAAKYKGILGINIGKNFDTPVERAVDDYLICLDKVYAHASYVTVNVSSPNTPGLRSLQFGDSLKQLLEALRQRQEDLAVRHGKRVPLAIKIAPDMSDEETVLVAQALVDSGMDAVIATNTTLSRVGVEGLAHGDEAGGLSGAPVRDKSTHTVKVLAAELAGRLPIIAVGGITEGKHAAEKIAAGASLVQLYTGFIYKGPALIRESVD |
Sequence similarities
Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length339
- Mass (Da)35,776
- Last updated2017-12-20 v1
- ChecksumDA38D056A9FFC4B7