A0A263NKD6 · A0A263NKD6_9PSED

  • Protein
    Phosphomethylpyrimidine synthase
  • Gene
    thiC
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site238substrate
Binding site267substrate
Binding site296substrate
Binding site332substrate
Binding site352-354substrate
Binding site393-396substrate
Binding site432substrate
Binding site436Zn2+ (UniProtKB | ChEBI)
Binding site459substrate
Binding site500Zn2+ (UniProtKB | ChEBI)
Binding site580[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site583[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site588[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functioncarbon-carbon lyase activity
Molecular Functionzinc ion binding
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphomethylpyrimidine synthase
  • EC number
  • Alternative names
    • Hydroxymethylpyrimidine phosphate synthase
      (HMP-P synthase
      ; HMP-phosphate synthase
      ; HMPP synthase
      )
    • Thiamine biosynthesis protein ThiC

Gene names

    • Name
      thiC
    • ORF names
      B7453_28760

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • IB20
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0A263NKD6

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain29-106ThiC-associated

Sequence similarities

Belongs to the ThiC family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    634
  • Mass (Da)
    70,376
  • Last updated
    2017-12-20 v1
  • Checksum
    4888DD9F719AE8BA
MSTEIKSTKLKNTVHLSESAKVDSGSVQPFTRSQKIYVQGTRPDIRVPMREISLDVTPTDLGGEINAPVVVYDTSGPYTDPNVIIDVRKGLADIRSPWIEERGDTERLAGLSSHYGRERLNDPELASLRFAHLQNPRRAKAGANVSQMHYARKGIITPEMEFVAIRENMKLEEARANGLLEQQHPGHSFGASVPKIITPEFVREEIARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVAKMTWGIRWGSDNIMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGAAEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFAYTHFEEICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKHDVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDDALSKARFEFRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRIEAVDVDVAKGLAEQAQRFKQEGSQLYKKV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NMRE01000162
EMBL· GenBank· DDBJ
OZO01084.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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