A0A249SVV7 · A0A249SVV7_9BACT

  • Protein
    ATP-dependent 6-phosphofructokinase
  • Gene
    pfkA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site14ATP (UniProtKB | ChEBI)
Binding site24-28ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners
Binding site75-76ATP (UniProtKB | ChEBI)
Binding site105-108ATP (UniProtKB | ChEBI)
Binding site106Mg2+ (UniProtKB | ChEBI); catalytic
Binding site129-131substrate; ligand shared between dimeric partners; in other chain
Active site131Proton acceptor
Binding site158ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site166substrate; ligand shared between dimeric partners
Binding site173-175substrate; ligand shared between dimeric partners; in other chain
Binding site189-191ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site215ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site217-219ADP (UniProtKB | ChEBI); allosteric activator; ligand shared between dimeric partners; in other chain
Binding site226substrate; ligand shared between dimeric partners; in other chain
Binding site250substrate; ligand shared between dimeric partners
Binding site256-259substrate; ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Component6-phosphofructokinase complex
Molecular Function6-phosphofructokinase activity
Molecular FunctionAMP binding
Molecular FunctionATP binding
Molecular Functionfructose-6-phosphate binding
Molecular Functionidentical protein binding
Molecular Functionmetal ion binding
Molecular Functionmonosaccharide binding
Biological Processcanonical glycolysis
Biological Processfructose 1,6-bisphosphate metabolic process
Biological Processfructose 6-phosphate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent 6-phosphofructokinase
  • EC number
  • Short names
    ATP-PFK
    ; Phosphofructokinase
  • Alternative names
    • Phosphohexokinase

Gene names

    • Name
      pfkA
    • ORF names
      CK934_12820

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • MD30
  • Taxonomic lineage
    Bacteria > Bacteroidota > Chitinophagia > Chitinophagales > Chitinophagaceae > Chitinophaga

Accessions

  • Primary accession
    A0A249SVV7

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain6-282Phosphofructokinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    326
  • Mass (Da)
    35,747
  • Last updated
    2017-12-20 v1
  • MD5 Checksum
    2E89F333C8754697C9E5ED41BF595E7B
MKKLNNIAVLTSGGDAPGMNAAVRAVVRTGIYHQLNVYGIMYGYRGMLKNEIFPMESKSVANIIQRGGTILKTARCKEFFEYEGRQKAYENLRKHNIDGLVVIGGDGSFNGAQKFSQEFDIPCIGLPGTIDKDIAGTDFTIGFDTAVNTAIEAIDKIRDTADAHDRLFVIEVMGRDAGYIALHSGLATGAEHIMLPENKIHVDDIIEDLQANERRRKLVNLIVVAEGHDTGGANEVARMVKEKCPQLDTRVCILGHIQRGGSPSCQDRILASRLGYAAVDALLQGIHNVMIGVVNDKIEYTPLDKAVKAKQQIDPEWFKIVKILAS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP023254
EMBL· GenBank· DDBJ
ASZ11776.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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