A0A240FW58 · A0A240FW58_9ALPC

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site5259
Active site5261
Active site5360
Active site5436
Active site5441
Binding site5499-5505S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site5914
Active site5929
Active site5970

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral genome replication
Biological Processviral protein processing
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Organism names

  • Taxonomic identifier
  • Strain
    • Wencheng-562
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus

Accessions

  • Primary accession
    A0A240FW58

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1541-1559Helical
Transmembrane1606-1627Helical
Transmembrane1670-1691Helical
Transmembrane1698-1716Helical
Transmembrane2089-2112Helical
Transmembrane2344-2371Helical
Transmembrane2383-2413Helical
Transmembrane2425-2456Helical
Transmembrane2873-2894Helical
Transmembrane2900-2919Helical
Transmembrane2926-2948Helical
Transmembrane2960-2980Helical
Transmembrane2992-3017Helical
Transmembrane3023-3043Helical
Transmembrane3050-3070Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1646↔1652

Keywords

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain112-196CoV Nsp2 N-terminal
Domain554-669Ubiquitin-like
Domain944-1113Macro
Domain1280-1536Peptidase C16
Domain1612-16773Ecto
Region1746-1836Y1
Domain1746-2084CoV Nsp3 Y
Region1750-1763ZF1
Region1796-1806ZF2
Region1837-2084CoV-Y
Region1983-2084Y4
Domain2468-2563Nsp4C
Domain2564-2863Peptidase C30
Domain3130-3211RdRp Nsp7 cofactor
Domain3212-3404RdRp Nsp8 cofactor
Domain3405-3513Nsp9 ssRNA-binding
Domain3514-3654ExoN/MTase coactivator
Domain3656-3906NiRAN
Domain3912-4010Nsp12 Interface
Domain4011-4577Nsp12 RNA-dependent RNA polymerase
Domain4578-4661CV ZBD
Domain4821-5185+RNA virus helicase C-terminal
Domain5241-5455ExoN
Domain5464-5685N7-MTase
Region5576-5590GpppA-binding
Domain5689-5749Nsp15 N-terminal oligomerization
Domain5750-5867AV-Nsp11N/CoV-Nsp15M
Domain5884-6024NendoU
Domain6027-6322Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,322
  • Mass (Da)
    716,856
  • Last updated
    2017-10-25 v1
  • Checksum
    985CE9036E4DF070

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KY967735
EMBL· GenBank· DDBJ
ASF90495.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help