A0A240FW30 · A0A240FW30_9ALPC

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site5257
Active site5259
Active site5358
Active site5434
Active site5439
Binding site5497-5503S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site5912
Active site5927
Active site5968

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral genome replication
Biological Processviral protein processing
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Organism names

  • Taxonomic identifier
  • Strain
    • Yudu-19
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus

Accessions

  • Primary accession
    A0A240FW30

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1539-1557Helical
Transmembrane1604-1625Helical
Transmembrane1684-1707Helical
Transmembrane2087-2110Helical
Transmembrane2342-2369Helical
Transmembrane2381-2411Helical
Transmembrane2423-2454Helical
Transmembrane2871-2892Helical
Transmembrane2898-2917Helical
Transmembrane2924-2946Helical
Transmembrane2958-2978Helical
Transmembrane2990-3015Helical
Transmembrane3046-3068Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1644↔1650

Keywords

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain112-196CoV Nsp2 N-terminal
Domain554-669Ubiquitin-like
Domain943-1112Macro
Domain1278-1534Peptidase C16
Domain1610-16753Ecto
Region1744-1834Y1
Domain1744-2082CoV Nsp3 Y
Region1748-1761ZF1
Region1794-1804ZF2
Region1835-2082CoV-Y
Region1981-2082Y4
Domain2466-2561Nsp4C
Domain2562-2861Peptidase C30
Domain3128-3209RdRp Nsp7 cofactor
Domain3210-3402RdRp Nsp8 cofactor
Domain3403-3511Nsp9 ssRNA-binding
Domain3512-3651ExoN/MTase coactivator
Domain3654-3904NiRAN
Domain3910-4008Nsp12 Interface
Domain4009-4575Nsp12 RNA-dependent RNA polymerase
Domain4576-4659CV ZBD
Domain4823-5183+RNA virus helicase C-terminal
Domain5239-5453ExoN
Domain5462-5683N7-MTase
Region5574-5588GpppA-binding
Domain5687-5747Nsp15 N-terminal oligomerization
Domain5748-5865AV-Nsp11N/CoV-Nsp15M
Domain5882-6022NendoU
Domain6025-6320Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,320
  • Mass (Da)
    716,160
  • Last updated
    2017-10-25 v1
  • Checksum
    3941DAD2A5D25012

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KY967724
EMBL· GenBank· DDBJ
ASF90464.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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