A0A231VMV9 · A0A231VMV9_THETR

Function

function

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site59-63(6S)-NADPHX (UniProtKB | ChEBI)
Binding site60K+ (UniProtKB | ChEBI)
Binding site131K+ (UniProtKB | ChEBI)
Binding site135-141(6S)-NADPHX (UniProtKB | ChEBI)
Binding site164(6S)-NADPHX (UniProtKB | ChEBI)
Binding site167K+ (UniProtKB | ChEBI)
Binding site264(6S)-NADPHX (UniProtKB | ChEBI)
Binding site334(6S)-NADPHX (UniProtKB | ChEBI)
Binding site383(6S)-NADPHX (UniProtKB | ChEBI)
Binding site420-424AMP (UniProtKB | ChEBI)
Binding site449AMP (UniProtKB | ChEBI)
Binding site450(6S)-NADPHX (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionADP-dependent NAD(P)H-hydrate dehydratase activity
Molecular FunctionATP binding
Molecular Functionkinase activity
Molecular Functionmetal ion binding
Molecular FunctionNADHX epimerase activity
Molecular FunctionNADPHX epimerase activity
Biological Processmetabolite repair
Biological Processnicotinamide nucleotide metabolic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional NAD(P)H-hydrate repair enzyme
  • Alternative names
    • Nicotinamide nucleotide repair protein

Including 2 domains:

  • Recommended name
    ADP-dependent (S)-NAD(P)H-hydrate dehydratase
  • EC number
  • Alternative names
    • ADP-dependent NAD(P)HX dehydratase
  • Recommended name
    NAD(P)H-hydrate epimerase
  • EC number

Gene names

    • Name
      nnrE
    • Synonyms
      nnrD
    • ORF names
      CE561_00985
      , Thert_02160

Organism names

Accessions

  • Primary accession
    A0A231VMV9

Proteomes

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain9-221YjeF N-terminal
Domain229-508YjeF C-terminal

Sequence similarities

Belongs to the NnrD/CARKD family.
Belongs to the NnrE/AIBP family.
In the C-terminal section; belongs to the NnrD/CARKD family.
In the N-terminal section; belongs to the NnrE/AIBP family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    508
  • Mass (Da)
    54,682
  • Last updated
    2017-10-25 v1
  • Checksum
    39427EE315BF373D
MKIVSNKEMRDIESMAINVVGVPSICLMENAGRIVAEHSKEYLLENKKNNVIILCGKGNNGGDGYVAARYLFNSGFEVKVFIIASIGTITGDAKKNLDIIVNMGIFVAEILQKEQLKFFEKNIKECDLIIDALYGTGLDREITGIGKDLIEIINGSGKYIISIDIPSGINGDNGHVEGCAVKANKTITMQFMKKGIAVYPGVDYAGDVIVADIGIPKNLIDSFNCKYHIVSQEDVNLPRRSRNTHKGDYGKLLIIAGSKNMTGAAALCAMSAIKTGCGIVKLAVPKTIQPILQESLKEIITYGVDDKDGAFYIGSVEEVLDLSKTVDAVAIGPGLSNSVDVKAFVKEIILRIDKPIVLDADALNVLSDMVDIIYGKDIILTPHMGEMSRLLGISIDDINKNIYDTVEKFVDKYKVTLVLKGSRTVIGSNTEGIYINCTGNPGMATAGSGDVLTGMISSFLVQGLTSIKAAVYGVFYHGKAGDLAADKYGEYGVTATNIIEHIPYAIKL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP016893
EMBL· GenBank· DDBJ
AST58095.1
EMBL· GenBank· DDBJ
Genomic DNA
NKHD01000003
EMBL· GenBank· DDBJ
OXT09419.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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