A0A228Q176 · A0A228Q176_9BURK
- ProteinShikimate dehydrogenase (NADP(+))
- GenearoE
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids277 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).
Catalytic activity
- NADP+ + shikimate = 3-dehydroshikimate + H+ + NADPH
Pathway
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 15-17 | shikimate (UniProtKB | ChEBI) | ||||
Sequence: TKS | ||||||
Binding site | 65 | shikimate (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Active site | 69 | Proton acceptor | ||||
Sequence: K | ||||||
Binding site | 81 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 90 | shikimate (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 105 | shikimate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 130-134 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GAGGA | ||||||
Binding site | 219 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 221 | shikimate (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 243 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 250 | shikimate (UniProtKB | ChEBI) | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | NADP binding | |
Molecular Function | shikimate 3-dehydrogenase (NADP+) activity | |
Biological Process | amino acid biosynthetic process | |
Biological Process | aromatic amino acid family biosynthetic process | |
Biological Process | chorismate biosynthetic process | |
Biological Process | shikimate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameShikimate dehydrogenase (NADP(+))
- EC number
- Short namesSDH
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Burkholderia
Accessions
- Primary accessionA0A228Q176
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 7-91 | Shikimate dehydrogenase substrate binding N-terminal | ||||
Sequence: VIGNPIGHTKSPLIHGLFAQETRQDIRYTAIEGPLEPAGAFAAAVRAFFDGGGKGINVTAPFKLDAFSMSDECSERAQLAGAANA | ||||||
Domain | 121-197 | Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase | ||||
Sequence: MAGKRVLVLGAGGAARGALLPFLEAGPAELVIANRDVDKACALATQVAGRGPLVAGSYADLARMGRFDLVVNATSAS | ||||||
Domain | 243-269 | SDH C-terminal | ||||
Sequence: GVGMLVEQAAEAFAWWRGVRPATSSVI |
Sequence similarities
Belongs to the shikimate dehydrogenase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length277
- Mass (Da)28,862
- Last updated2017-10-25 v1
- Checksum35EF479AE5F2E361