A0A226Q471 · A0A226Q471_9BACI

Function

function

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ cation per monomer.
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Note: Binds 1 nickel ion per monomer.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site99Fe2+ (UniProtKB | ChEBI)
Binding site99Ni2+ (UniProtKB | ChEBI)
Binding site101Fe2+ (UniProtKB | ChEBI)
Binding site101Ni2+ (UniProtKB | ChEBI)
Site104May play a role in transmitting local conformational changes
Binding site105Fe2+ (UniProtKB | ChEBI)
Binding site105Ni2+ (UniProtKB | ChEBI)
Site107Important to generate the dianion
Binding site145Fe2+ (UniProtKB | ChEBI)
Binding site145Ni2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionacireductone dioxygenase (Ni2+-requiring) activity
Molecular Functionacireductone dioxygenase [iron(II)-requiring] activity
Molecular Functioniron ion binding
Molecular Functionnickel cation binding
Biological ProcessL-methionine salvage from methylthioadenosine
Biological ProcessL-methionine salvage from S-adenosylmethionine

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Acireductone dioxygenase
  • Alternative names
    • 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
      (DHK-MTPene dioxygenase
      )
    • Acireductone dioxygenase (Fe(2+)-requiring)
      (ARD'
      ; Fe-ARD
      ) (EC:1.13.11.54
      ) . EC:1.13.11.54 (UniProtKB | ENZYME | Rhea)
    • Acireductone dioxygenase (Ni(2+)-requiring)
      (ARD
      ; Ni-ARD
      ) (EC:1.13.11.53
      ) . EC:1.13.11.53 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      mtnD
    • ORF names
      B9L19_13555

Organism names

  • Taxonomic identifier
  • Strain
    • BGSC 93A1
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Geobacillus > Geobacillus thermoleovorans group

Accessions

  • Primary accession
    A0A226Q471

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Sequence similarities

Belongs to the acireductone dioxygenase (ARD) family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    180
  • Mass (Da)
    20,592
  • Last updated
    2017-10-25 v1
  • Checksum
    BB43E12CACF32BC5
MAVIKVRKTGQVIEGEDNVRAFLNSQGVLYEHWDITKLPEHLRDKYVLTDEEKNEILAAFKDEIEDLAARRGYKTWDIVALSDATPNLDELLKKFEQVHIHTEDEVRAITAGHGIFIIKGDKETGYFDVELEAGDVISVPEGNPHYFTLMDDRRVVAVRLFIDPSGWVAHPYEEKEEAVQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NEWK01000002
EMBL· GenBank· DDBJ
OXB86547.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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