A0A225CMT9 · A0A225CMT9_9MICO
- ProteinMethionine--tRNA ligase
- GenemetG
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids525 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
Catalytic activity
- ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | methionine-tRNA ligase activity | |
Biological Process | methionyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethionine--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micrococcales > Microbacteriaceae > Clavibacter
Accessions
- Primary accessionA0A225CMT9
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 8-368 | Methionyl/Leucyl tRNA synthetase | ||||
Sequence: YITTPIFYVNDVPHIGHAYTEVAADVLARWHRQRGDDTWFLTGTDEHGQKILRTATANDTTPQAWADRLVTESWQPLLAAVDISNDDFIRTTDARHEESVKVFLQRLHDAGYIYTGEYKGYYCVGCEEYKQPSDLLEGTGPFEGQLVCAIHSKPVELLEEKNYFFRMSDFGERLLAFYEERPDFIQPESARNEILSFVRRGLEDLSISRSSFDWGIPIPWDESHVVYVWFEALMNYVTAIGYGVDDEEFRRRWPATHLVGKDILRFHAVIWPAMLMALGEEPPRRVFGHGWLLVGGEKMSKSKLTGIVPQTITDTFGIDAFRYHFMRAFAFGQDGSFSWEDLSARYQAELANGFGNLSSRV | ||||||
Motif | 14-24 | 'HIGH' region | ||||
Sequence: FYVNDVPHIGH | ||||||
Motif | 305-309 | 'KMSKS' region | ||||
Sequence: KMSKS | ||||||
Domain | 391-492 | Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding | ||||
Sequence: ERVLSVARSAAETADEAIERLAIHEALAAVWTLVDELNGYITSQEPWALAKKEDGRARLETVLHTAVRGLGTLAVLLAPVLPGATAKLWTALGGTGTVGQQR |
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length525
- Mass (Da)58,890
- Last updated2017-10-25 v1
- Checksum690B1421CEB5BAD5