A0A223NQK8 · A0A223NQK8_9SPHI

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

111261002003004005006007008009001,0001,100
TypeIDPosition(s)Description
Binding site680ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      MuYL_0214

Organism names

  • Taxonomic identifier
  • Strain
    • BJC16-A31
  • Taxonomic lineage
    Bacteria > Bacteroidota > Sphingobacteriia > Sphingobacteriales > Sphingobacteriaceae > Mucilaginibacter

Accessions

  • Primary accession
    A0A223NQK8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain17-710Aminoacyl-tRNA synthetase class Ia
Motif47-57'HIGH' region
Motif677-681'KMSKS' region
Domain764-917Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,126
  • Mass (Da)
    128,409
  • Last updated
    2017-10-25 v1
  • Checksum
    21DCE439422F2877
MYKEYKQLNLSQTGKDVLDFWKQNNIFEKSISSRPANNPYTFYEGPPSANGMPGIHHVMARAIKDIFCRYKTLKGYQVKRKGGWDTHGLPIELAVEKALGITKDDIGKTISVKDYNDACRKEVMRYTDIWNDLTEKMGYWVDLENPYITYKNEYIESLWWILKEFHTKGLLYKGYTVQPYSPKSGTGLSSHELNQPGTYKMVKDTSITAQFKVKRDKDSEFLFEGVDTDLYILAWTTTPWTLPANCALAVGEKIDYVKISTFNPYTYEPVTVILANDLVGKHFKAEGENAFFDDYKGGDKIIPWEVKGHYKGTDLLGLHYWQLMPYVTNENLEKNAFRVIPADFVTTEDGTGIVHTASVFGADDFRACKENGVPSVMVYDEFGKEVPLVNKQGRFVDEVTDFAGLYVKEEYYSKEEREAPGFKATDVLISIKLKTENKAFDVKKYEHSYPHSWRSDEPILYYPLDSWFIKTTAVKDKMVALNKTINWKPESTGTGRFGNWLENLVDWNLSRSRFWGTPLPIWREENGGEEKCIGSIADLTAEIEKSIAAGLMPAGFVIDDLHRPYVDDVILTSSTGKPMYREPDLIDVWFDSGAMPYAQWHFPFENKEEFKNAYPADFIAEGVDQTRGWFFTLHAIAVMLSETSDEIKKINEEVGNKGVAFKNVISNGLVLDKNGNKMSKRLGNGVDPFETIEKFGADAPRWYMISNAAPWDNLKFNPEGIDEVRRKFFGTLYNTYTFFVMYANIDGFTYSEAEIALKDRPEIDQWIISLLNTLSKEVDAFYADFEPTKAARAIQDFVDAHLSNWYVRLSRRRFWRSDSPQDKLSAYQTLYTCLTTIAKLMSPIAPFFAERLYTDLNSITQKEEFESVHLAYFPEYHPDLVNAELEERMQMAQDISSLVLSLRKKVSINVRQPLSKILLPILDKKFEGQIEQVKDLILSETNIKAIEYITDTAGFIKKKIKPNFKALGAKVGKDMKAVAEAIGNLSQDDIAALENNGDLLLPTRHLINLSDVEIIAEDVTGWQVANLGKLTVALDVTITDELKQEGISRELINRIQNLRKGKEFEVTDRINVKLSNHPFISKAVNNNLSYICAEILAESIVLDNELNEGELVEIDGNEILIAISKI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP022743
EMBL· GenBank· DDBJ
ASU32117.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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