A0A223D6A2 · A0A223D6A2_9BACL
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids670 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 74-75 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: SL | ||||||
Binding site | 115 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 117 | N6-AMP-lysine intermediate | ||||
Sequence: K | ||||||
Binding site | 138 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 172 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 289 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 313 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 406 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 409 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 422 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 428 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | DNA binding | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, DNA ligation | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Alicyclobacillaceae > Tumebacillus
Accessions
- Primary accessionA0A223D6A2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 591-670 | BRCT | ||||
Sequence: AAESPFFGKTVVLTGTLTMMGRNEAKAKLEAFGAKVGSSVSKKTDFVIAGAEAGSKLTKAQDLGVAVLTEQEFLAVIGEA |
Sequence similarities
Belongs to the NAD-dependent DNA ligase family. LigA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length670
- Mass (Da)74,257
- Last updated2017-10-25 v1
- ChecksumE8BC650FCF195BA3
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP022657 EMBL· GenBank· DDBJ | ASS77101.1 EMBL· GenBank· DDBJ | Genomic DNA |