A0A219YHE1 · A0A219YHE1_BPK54

Function

function

ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination. The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis. ATP or dTTP hydrolysis propels each helicase domain to translocate sequentially along DNA. Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange. Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+, one of which is catalytic.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site40Zn2+ (UniProtKB | ChEBI)
Binding site43Zn2+ (UniProtKB | ChEBI)
Binding site59Zn2+ (UniProtKB | ChEBI)
Binding site62Zn2+ (UniProtKB | ChEBI)
Binding site184Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site234Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site264Mg2+ 2 (UniProtKB | ChEBI)
Binding site339-346ATP (UniProtKB | ChEBI)
Site388dTTP/dATP binding
Site493dTTP/dATP binding
Site532dTTP/dATP binding
Site550dTTP/dATP binding
Site564dTTP/dATP binding

GO annotations

AspectTerm
Cellular Componentprimosome complex
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionDNA primase activity
Molecular Functionhydrolase activity
Molecular Functionsingle-stranded DNA binding
Molecular Functionzinc ion binding
Biological Processviral DNA genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • ORF names
      k54_015

Organism names

Accessions

  • Primary accession
    A0A219YHE1

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homohexamer. Assembles as a hexamer onto linear or circular ssDNA in the presence of ATP or dTTP. Interacts (via C-terminus) with the viral DNA polymerase that is bound to DNA; this interaction is essential to initiate leading-strand DNA synthesis. The priming complex consists of 2 DNA polymerases and 1 helicase-primase hexamer that assemble on the DNA template. Interacts with the single-stranded DNA-binding protein. Part of the replicase complex that includes the DNA polymerase, the primase/helicase and the single-stranded DNA binding protein.

Family & Domains

Features

Showing features for zinc finger, domain, region.

TypeIDPosition(s)Description
Zinc finger40-62C4-like; zinc ribbon fold
Domain178-265Toprim
Domain308-577SF4 helicase
Region571-595Disordered

Domain

The N-terminus zinc finger domain is essential for delivering the primed DNA template to the DNA polymerase. The central core domain contains the primase activity. The C-terminus region is responsible for the helicase activity and binds 1 Mg2+-dTTP.

Sequence similarities

Belongs to the Teseptimavirus DNA helicase/primase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    595
  • Mass (Da)
    65,738
  • Last updated
    2017-10-25 v1
  • Checksum
    02ED9965314F1346
MGAVLRGLVYHFMHQRYIVMSYGDSQEDGQESIFLFHAPCENCGSSDGNSVYSDGHEYCFVCQHRVRGSEERTEKLSSRRPKGGNYGMNTQGSGLLVFGESDGRYTDLTARGISKATCQKAGYWVAKVRGTAYQVADYRDQNGSIVSQKLRDKEKNFSTRGSHKGDALFGKHLWNGGKKIVITEGEIDMLTVMQLQDCKWPVVSLGHGASAAKKTCSANYEYFDSFDQIILMFDMDDPGRAAVEEAAQVLPPGKVHVAVLTEKDANECLLKGKGKEVLDQIWNAAPWVPDGVIGAMSMKDRVREAMTSEQSVGYLFSGCPGLNDRTLGARGGEVIMVTSGSGMGKSTFVRQQALGFARGQGLRVGMAMLEESVEETMEDVLGIANGIRLRQQPREFKQKLIEDGTYDKWFDELYGSDQFHLYDSFAEAEVDRLLAKLHYMRTGLNCDVIILDHISIVVSASEESDERKMIDRLMTKLKGFAKSTGVVLIVICHLKNPEKGKAHEEGRAVSITDLRGSGSLRQLSDTIIALERNQQGDMPNLVLLRILKCRFNGIGVGIAGYMEYNEKTGLLEPSSYTGGEGEGDTGWEGHEEDDY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KY389316
EMBL· GenBank· DDBJ
APZ82825.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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