A0A218Q112 · A0A218Q112_9CYAN

  • Protein
    (S)-8-amino-7-oxononanoate synthase BioU
  • Gene
    bioU
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

A 'suicide' enzyme that participates in biotin synthesis. Catalyzes the formation of (S)-8-amino-7-oxononanoate (DAN-carbamic acid) from (7R,8S)-8-amino-7-(carboxyamino)nonanoate (DAN), a function equivalent to the cannonical BioA reaction and the first half-reaction of BioD. The cellular requirement for biotin is thought be low enough that this single turnover enzyme supplies a sufficient amount of the cofactor. Overall it catalyzes three reactions: formation of a covalent linkage with 8-amino-7-oxononanoate to yield a BioU-DAN conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-DAN-carbamic acid, and release of DAN-carbamic acid using NAD(P)+.

Miscellaneous

In cannonical biotin synthesis a pimeloyl-conjugate is transformed into biotin by the subsequent action of BioF, BioA, BioD and BioB. This enzyme replaces BioA and performs the first half-reaction of BioD.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; biotin biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site16-20NAD+ (UniProtKB | ChEBI)
Binding site61NAD+ (UniProtKB | ChEBI)
Active site127Nucleophile
Binding site193-194NAD+ (UniProtKB | ChEBI)
Active site197Proton acceptor
Active site201Proton donor and proton acceptor

GO annotations

AspectTerm
Molecular FunctionNAD binding
Molecular FunctionNADP binding
Molecular Functionoxidoreductase activity
Molecular Functiontransaminase activity
Biological Processamino acid metabolic process
Biological Processbiotin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    (S)-8-amino-7-oxononanoate synthase BioU
  • EC number
  • Alternative names
    • 8-amino-7-oxononanoate carboxylating dehydrogenase

Gene names

    • Name
      bioU
    • ORF names
      NIES3585_15790

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • NIES-3585
  • Taxonomic lineage
    Bacteria > Cyanobacteriota > Cyanophyceae > Nostocales > Aphanizomenonaceae > Nodularia

Accessions

  • Primary accession
    A0A218Q112

Proteomes

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue127Allysine

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain11-95Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal

Sequence similarities

Belongs to the BioU family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    334
  • Mass (Da)
    35,750
  • Last updated
    2017-09-27 v1
  • Checksum
    95579AC9DC9BF0E9
MNTQPTNQPIRVGVLGFGGLGQAAAKLLSSKREMILVAAADQKGYAYSTEGLNTQECIKTYQSQGSVGYLEPVGRLSNNSIQDLIATTQPVDGYFLALPNLPNDFIPSVAQQFIKSGWRGVLVDAIKRTSAVEQLLAMKDELQAAGITYMSGCGATPGLLTAAAALAAQSYAEIHKVEITFGVGIANWEAYRATVREDIGHMPGYSVETARAMTDAEVEALLDKTNGVLTLDNMEHADDVMLEFAGICSRDRVTVGGVVDTRNAKKPLSTNVKVTGRTFEGKISTHTFTLGDETSMAANVCGPAFGYLKAGRQLHQRGIYGIFTAAEIMPQFVK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BDUB01000001
EMBL· GenBank· DDBJ
GAX35562.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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