A0A218PNZ0 · A0A218PNZ0_9HYPH

  • Protein
    Bifunctional enzyme IspD/IspF
  • Gene
    ispDF
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site15Transition state stabilizer
Site24Transition state stabilizer
Site155Positions MEP for the nucleophilic attack
Site212Positions MEP for the nucleophilic attack
Binding site241a divalent metal cation (UniProtKB | ChEBI)
Binding site241-2434-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site243a divalent metal cation (UniProtKB | ChEBI)
Site267Transition state stabilizer
Binding site267-2684-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site275a divalent metal cation (UniProtKB | ChEBI)
Binding site289-2914-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site365-3684-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Site366Transition state stabilizer
Binding site3724-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site3754-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Molecular Function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
Molecular Functionmetal ion binding
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional enzyme IspD/IspF

Including 2 domains:

  • Recommended name
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • EC number
  • Alternative names
    • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    • MEP cytidylyltransferase
      (MCT
      )
  • Recommended name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • EC number
  • Short names
    MECDP-synthase
    ; MECPP-synthase
    ; MECPS

Gene names

    • Name
      ispDF
    • ORF names
      HYPGJ_20719

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • GJ21
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Hyphomicrobiaceae > Hyphomicrobium

Accessions

  • Primary accession
    A0A218PNZ0

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-2342-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Domain235-3872-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region235-3952-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Sequence similarities

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.
Belongs to the IspF family.
In the C-terminal section; belongs to the IspF family.
In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    395
  • Mass (Da)
    41,594
  • Last updated
    2017-09-27 v1
  • Checksum
    A1BB0DA5A420A67E
MRIAALIVAAGRGTRAAASGAGPKQYALIGGRPVLAHTIAAFADHPEIDEIKVVIHADDGELYAHASGHAAKLSSAATGGATRQASVHNGLEALAGSKPDLVLIHDAARPFVSAETISKVIATLKSTRAALAALPVTDTLKRASDRGVVEDTIPRAGLWRAQTPQGFHFDPILEAHRKAAEQGLDKFTDDAAIAEWAGFDVAIVEDETGNIKLTTAEDLKLADRQMTSNIAFEPRTGTGFDVHRFTEGDHVWLGGVKIPHTHKLEGHSDADVILHALTDALLGAIGDGDIGQHFPPSDPKWKGAASRLFLEDAARRVKDRGGRIVNVDITVLAEAPRVGPHRPAMQALIGDVLGLTTDRIGIKATTTEGMGFTGRREGIAAMASASVLLPYGLSE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CDHO01000002
EMBL· GenBank· DDBJ
CEJ85031.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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