A0A218PB86 · A0A218PB86_9EURY

  • Protein
    DNA topoisomerase 1
  • Gene
    topA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

170350100150200250300350400450500550600650700
TypeIDPosition(s)Description
Site53Interaction with DNA
Site167Interaction with DNA
Site171Interaction with DNA
Active site318O-(5'-phospho-DNA)-tyrosine intermediate
Site320Interaction with DNA
Site508Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      A3L12_01975

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • P6
  • Taxonomic lineage
    Archaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Thermococcus

Accessions

  • Primary accession
    A0A218PB86

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain2-142Toprim
Region197-202Interaction with DNA
Region363-382Disordered
Region595-617Disordered
Compositional bias598-614Basic and acidic residues

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    703
  • Mass (Da)
    79,744
  • Last updated
    2017-09-27 v1
  • Checksum
    F8DED511378412F8
MVTLIIAEKPNVARKIAYALAEGKPVRKTIGKVSYYEFTRDGKRLVVAPAVGHLFSLAPKSRTYGYPVFDVEWVPVYVAERGKGYARDYIKALAHLAKGADEFVVACDYDTEGEVIGYTALKYACGVDPARAKRMRFSALTKKDLLRAWYNLEPTINFGMADAGIARHVLDWYWGVNLSRALTSSIKRASGKWMVLSTGRVQGPTLKFLVDREREIANFRPTPYWVVKMLLEKNGENYTATYERERILDEAEAKRIVEESKKGPAFVERVEVRRGKRHPPVPFDLGTLQREAYSAFKYSPKKTLDLAQKLYEKGLSSYPRTSSQKLPSSLNFRSILKGLAGLTEYRPFAHELLGREVLKPVQGKKDDPAHPAIYPTGELPKEGELTKDERNLYDLIVRRFLALFMEPAIRENVRVVINSNSHRFILSGARTVSEGWLKVYGKYVRFDEVILPAFKEGEPVKVLGIRREKKKTKPPSRYSPAAVIKRMEDLGIGTKATRAQILETLYSRGYIEGKRKIKVTPLGMRVVEALEKNVPDIVSVELTRAFEEKMEDVTVGKERKEKVIEESRNRLIKILSVFRERELEIGRMLMEAAPTRKDGAVGDSENDGKGKTSGKKKPLVVGRCPRCGGDLVVRYNRKTGKRFVGCSNWPKCNVTYPLLQRGEIIPTDKTCCGGAPVVRIREKGREYEVCLDMNCRDWKKDKK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias598-614Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP015104
EMBL· GenBank· DDBJ
ASJ10149.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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