A0A218N6S8 · A0A218N6S8_ECOLX
- ProteinDiacylglycerol kinase
- GenedgkA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids126 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent phosphorylation of sn-l,2-diacylglycerol (DAG) to phosphatidic acid. Involved in the recycling of diacylglycerol produced as a by-product during membrane-derived oligosaccharide (MDO) biosynthesis.
Catalytic activity
- a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H+
Cofactor
Note: Mn2+, Zn2+, Cd2+ and Co2+ support activity to lesser extents.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 11 | ATP (UniProtKB | ChEBI) | |||
Binding site | 11 | substrate | |||
Binding site | 18 | ATP (UniProtKB | ChEBI) | |||
Binding site | 30 | ATP (UniProtKB | ChEBI) | |||
Binding site | 30 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 32-36 | substrate | |||
Active site | 71 | Proton acceptor | |||
Binding site | 71 | substrate | |||
Binding site | 78 | ATP (UniProtKB | ChEBI) | |||
Binding site | 78 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 87-89 | ATP (UniProtKB | ChEBI) | |||
Binding site | 96-97 | ATP (UniProtKB | ChEBI) | |||
Binding site | 100 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | ATP-dependent diacylglycerol kinase activity | |
Molecular Function | metal ion binding | |
Biological Process | phosphatidic acid biosynthetic process | |
Biological Process | phosphorylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDiacylglycerol kinase
- EC number
Gene names
Encoded on
- Plasmid pCHL5009T-102k-mcr3
- Plasmid pNCYU-26-73-3_MCR3
- Plasmid pWJ1
- Plasmid pchl5009t-102k-mcr3
- Plasmid pncyu-26-73-3_mcr3
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A218N6S8
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 31-51 | Helical | |||
Transmembrane | 98-117 | Helical | |||
Keywords
- Cellular component
Structure
Sequence
- Sequence statusComplete
- Length126
- Mass (Da)13,837
- Last updated2017-09-27 v1
- MD5 Checksum568FD6B1A6C19D849378C19219E9E497
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KY924928 EMBL· GenBank· DDBJ | ASF81897.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP032937 EMBL· GenBank· DDBJ | AYM25022.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BGDN01000026 EMBL· GenBank· DDBJ | GDP60496.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BGIE01000041 EMBL· GenBank· DDBJ | GDV31484.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP042618 EMBL· GenBank· DDBJ | QED76429.1 EMBL· GenBank· DDBJ | Genomic DNA |