A0A218LRM8 · A0A218LRM8_9POAL
- ProteinDNA-directed RNA polymerase subunit beta''
- GenerpoC2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1479 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta''
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Pooideae > Triticodae > Triticeae > Hordeinae > Elymus
Accessions
- Primary accessionA0A218LRM8
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 93-157 | RNA polymerase Rpb1 | ||||
Sequence: AVEKLRQSVEIWYATSEYLKQEMNSNFRITDPSNPVYLMSFSGARXXXXXXXXXXGMRGLMSDPQ | ||||||
Domain | 172-370 | RNA polymerase Rpb1 | ||||
Sequence: GLSLTEYIISCYGARKGVVDTAVRTADAGYLTRRLVEVVQHIIVRRRDCGTIRGISVSPQNGMTEKLFVQTLIGRVLADDIYIGSRCIAARNQDIGIGLVNRFITAFRAQPFRAQPIYIRTPFTCRSTSWICQLCYGRSPTHSDLVELGEAVGIIAGQSIGEPGTQLTLRTFHTGGVFTGGTADLVRSPSNGKIQFNEN | ||||||
Region | 620-756 | Disordered | ||||
Sequence: AEDSEEEYETLEDEYRTREDEYEYETLEESKYGILEDEYEYETLEDEYGSPENEYGNPENEYRTLEKDSEEEYGSPESKYRTQEDEYGTLEEDSEDEYGSPGESAEEKYGTLEEDSEEDSEDEYESPEEDSILKKEG | ||||||
Compositional bias | 634-657 | Basic and acidic residues | ||||
Sequence: YRTREDEYEYETLEESKYGILEDE | ||||||
Compositional bias | 678-706 | Basic and acidic residues | ||||
Sequence: ENEYRTLEKDSEEEYGSPESKYRTQEDEY | ||||||
Compositional bias | 729-746 | Acidic residues | ||||
Sequence: GTLEEDSEEDSEDEYESP | ||||||
Domain | 1292-1378 | RNA polymerase Rpb1 | ||||
Sequence: LTIAQSRISLVNKIQKVYRSQGVQIHNRHIEIIIRQVTSKVRVSEDGMSNVFSPGELIGLLRAERAGRALDESIYYRAILLGITRAS |
Sequence similarities
Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,479
- Mass (Da)170,236
- Last updated2017-09-27 v1
- Checksum23438B89D74B741C
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 634-657 | Basic and acidic residues | ||||
Sequence: YRTREDEYEYETLEESKYGILEDE | ||||||
Compositional bias | 678-706 | Basic and acidic residues | ||||
Sequence: ENEYRTLEKDSEEEYGSPESKYRTQEDEY | ||||||
Compositional bias | 729-746 | Acidic residues | ||||
Sequence: GTLEEDSEEDSEDEYESP |