A0A218LRM8 · A0A218LRM8_9POAL
- ProteinDNA-directed RNA polymerase subunit beta''
- GenerpoC2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1479 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta''
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Pooideae > Triticodae > Triticeae > Hordeinae > Elymus
Accessions
- Primary accessionA0A218LRM8
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 93-157 | RNA polymerase Rpb1 | ||||
Sequence: AVEKLRQSVEIWYATSEYLKQEMNSNFRITDPSNPVYLMSFSGARXXXXXXXXXXGMRGLMSDPQ | ||||||
Domain | 172-370 | RNA polymerase Rpb1 | ||||
Sequence: GLSLTEYIISCYGARKGVVDTAVRTADAGYLTRRLVEVVQHIIVRRRDCGTIRGISVSPQNGMTEKLFVQTLIGRVLADDIYIGSRCIAARNQDIGIGLVNRFITAFRAQPFRAQPIYIRTPFTCRSTSWICQLCYGRSPTHSDLVELGEAVGIIAGQSIGEPGTQLTLRTFHTGGVFTGGTADLVRSPSNGKIQFNEN | ||||||
Region | 620-756 | Disordered | ||||
Sequence: AEDSEEEYETLEDEYRTREDEYEYETLEESKYGILEDEYEYETLEDEYGSPENEYGNPENEYRTLEKDSEEEYGSPESKYRTQEDEYGTLEEDSEDEYGSPGESAEEKYGTLEEDSEEDSEDEYESPEEDSILKKEG | ||||||
Compositional bias | 634-657 | Basic and acidic residues | ||||
Sequence: YRTREDEYEYETLEESKYGILEDE | ||||||
Compositional bias | 678-706 | Basic and acidic residues | ||||
Sequence: ENEYRTLEKDSEEEYGSPESKYRTQEDEY | ||||||
Compositional bias | 729-746 | Acidic residues | ||||
Sequence: GTLEEDSEEDSEDEYESP | ||||||
Domain | 1292-1378 | RNA polymerase Rpb1 | ||||
Sequence: LTIAQSRISLVNKIQKVYRSQGVQIHNRHIEIIIRQVTSKVRVSEDGMSNVFSPGELIGLLRAERAGRALDESIYYRAILLGITRAS |
Sequence similarities
Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,479
- Mass (Da)170,236
- Last updated2017-09-27 v1
- Checksum23438B89D74B741C
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 634-657 | Basic and acidic residues | ||||
Sequence: YRTREDEYEYETLEESKYGILEDE | ||||||
Compositional bias | 678-706 | Basic and acidic residues | ||||
Sequence: ENEYRTLEKDSEEEYGSPESKYRTQEDEY | ||||||
Compositional bias | 729-746 | Acidic residues | ||||
Sequence: GTLEEDSEEDSEDEYESP |