A0A218LNL4 · A0A218LNL4_PSAJU
- ProteinDNA-directed RNA polymerase subunit beta''
- GenerpoC2
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1475 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 220 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 296 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 303 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 306 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta''
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Pooideae > Triticodae > Triticeae > Hordeinae > Psathyrostachys
Accessions
- Primary accessionA0A218LNL4
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain, coiled coil, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 93-157 | RNA polymerase Rpb1 | |||
Domain | 207-399 | RNA polymerase Rpb1 | |||
Coiled coil | 613-640 | ||||
Region | 658-752 | Disordered | |||
Compositional bias | 674-702 | Basic and acidic residues | |||
Compositional bias | 725-742 | Acidic residues | |||
Domain | 1288-1375 | RNA polymerase Rpb1 | |||
Sequence similarities
Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,475
- Mass (Da)169,843
- Last updated2017-09-27 v1
- Checksum0DC5E11DED01F493
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 674-702 | Basic and acidic residues | |||
Compositional bias | 725-742 | Acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KY636111 EMBL· GenBank· DDBJ | ASD42860.1 EMBL· GenBank· DDBJ | Genomic DNA |