A0A210W9Z4 · A0A210W9Z4_9BURK

  • Protein
    Acireductone dioxygenase
  • Gene
    mtnD
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ cation per monomer.
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Note: Binds 1 nickel ion per monomer.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site93Fe2+ (UniProtKB | ChEBI)
Binding site93Ni2+ (UniProtKB | ChEBI)
Binding site95Fe2+ (UniProtKB | ChEBI)
Binding site95Ni2+ (UniProtKB | ChEBI)
Site98May play a role in transmitting local conformational changes
Binding site99Fe2+ (UniProtKB | ChEBI)
Binding site99Ni2+ (UniProtKB | ChEBI)
Site101Important to generate the dianion
Binding site138Fe2+ (UniProtKB | ChEBI)
Binding site138Ni2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionacireductone dioxygenase (Ni2+-requiring) activity
Molecular Functionacireductone dioxygenase [iron(II)-requiring] activity
Molecular Functioniron ion binding
Molecular Functionnickel cation binding
Biological ProcessL-methionine salvage from methylthioadenosine
Biological ProcessL-methionine salvage from S-adenosylmethionine

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acireductone dioxygenase
  • Alternative names
    • 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
      (DHK-MTPene dioxygenase
      )
    • Acireductone dioxygenase (Fe(2+)-requiring)
      (ARD'
      ; Fe-ARD
      ) (EC:1.13.11.54
      ) . EC:1.13.11.54 (UniProtKB | ENZYME | Rhea)
    • Acireductone dioxygenase (Ni(2+)-requiring)
      (ARD
      ; Ni-ARD
      ) (EC:1.13.11.53
      ) . EC:1.13.11.53 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      mtnD
    • ORF names
      KDK82_2525

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • K82
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Delftia

Accessions

  • Primary accession
    A0A210W9Z4

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Sequence similarities

Belongs to the acireductone dioxygenase (ARD) family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    176
  • Mass (Da)
    19,128
  • Last updated
    2017-11-22 v1
  • Checksum
    D3C0C7615912E839
MGALASHRVSDTDFVAAIGVQWQHRPLAPEPGLAALLAQAQLDDGGKATVVQAVGMLVVDECVAHGYHSMDLVVLHPGTPGLDEALERFDKPHTHADDEVRYVLEGEGLFGFFDAHGQERVLRVQPGDYLRIPAGVEHRFTLTATRRIKALRLFADTAGWVAQYTQRPAAAMEVPA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MYFL01000001
EMBL· GenBank· DDBJ
OWG19043.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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