A0A210PZS6 · A0A210PZS6_MIZYE
- ProteinSuccinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids431 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity
- GTP + succinate + CoA = succinyl-CoA + GDP + phosphate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 58 | GTP (UniProtKB | ChEBI) | |||
Site | 80 | Important for substrate specificity | |||
Binding site | 91-93 | GTP (UniProtKB | ChEBI) | |||
Binding site | 147 | GTP (UniProtKB | ChEBI) | |||
Site | 148 | Important for substrate specificity | |||
Binding site | 244 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 258 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 309 | substrate; ligand shared with subunit alpha | |||
Binding site | 366-368 | substrate; ligand shared with subunit alpha | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | succinate-CoA ligase complex | |
Molecular Function | ATP binding | |
Molecular Function | GTP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | succinate-CoA ligase (ADP-forming) activity | |
Molecular Function | succinate-CoA ligase (GDP-forming) activity | |
Biological Process | succinyl-CoA metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Spiralia > Lophotrochozoa > Mollusca > Bivalvia > Autobranchia > Pteriomorphia > Pectinida > Pectinoidea > Pectinidae > Mizuhopecten
Accessions
- Primary accessionA0A210PZS6
Proteomes
Subcellular Location
Interaction
Subunit
Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 40-246 | ATP-grasp fold succinyl-CoA synthetase-type | |||
Domain | 307-425 | ATP-citrate synthase/succinyl-CoA ligase C-terminal | |||
Sequence similarities
Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length431
- Mass (Da)46,772
- Last updated2017-09-27 v1
- ChecksumF9FB30625C84EAC0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
NEDP02005326 EMBL· GenBank· DDBJ | OWF41982.1 EMBL· GenBank· DDBJ | Genomic DNA |