A0A210PXI6 · A0A210PXI6_MIZYE

Function

function

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of the sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site91ATP (UniProtKB | ChEBI)
Binding site112ATP (UniProtKB | ChEBI)
Binding site119-123ATP (UniProtKB | ChEBI)
Binding site136ATP (UniProtKB | ChEBI)
Binding site180-181ATP (UniProtKB | ChEBI)
Binding site221Zn2+ (UniProtKB | ChEBI)
Binding site224Zn2+ (UniProtKB | ChEBI)
Active site238Glycyl thioester intermediate; for adenylyltransferase activity
Binding site296Zn2+ (UniProtKB | ChEBI)
Binding site299Zn2+ (UniProtKB | ChEBI)
Active site404Cysteine persulfide intermediate; for sulfurtransferase activity

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionadenylyltransferase activity
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionthiosulfate sulfurtransferase activity
Molecular FunctionURM1 activating enzyme activity
Biological ProcessMo-molybdopterin cofactor biosynthetic process
Biological Processprotein urmylation
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Adenylyltransferase and sulfurtransferase MOCS3 homolog
  • Alternative names
    • UBA4 homolog
    • Ubiquitin-like protein activator 4 homolog

Including 2 domains:

  • Recommended name
    Adenylyltransferase
  • EC number
  • Recommended name
    Sulfurtransferase
  • EC number

Gene names

    • ORF names
      KP79_PYT09839

Organism names

Accessions

  • Primary accession
    A0A210PXI6

Proteomes

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain346-447Rhodanese

Sequence similarities

In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    449
  • Mass (Da)
    49,429
  • Last updated
    2017-09-27 v1
  • Checksum
    D5A97C0B5DDAF261
MMETEDSDLRKLLKEKDDEIERLKKLLTIKTPAVVEEKDIFVQSDDRPAVREKLNNAEISRYSRQLILPEIGVKGQLSISSASVLVVGAGGLGCPSSIYLAAAGIGRIGLVDYDEVELTNLHRQILHTEAKVGVTKSASAAHACKSLNSHVECIPYHLQLNSSNALQLIKQYDIVLDATDNVATRYLLNDACILANKPLVSGSALRFEGQLTVYNYDGGPCYRCLYPKPPPPETVTNCSDGGVLGVVPGTIGCLQALEAIKIAAGIGPSFSQVMLMFDGLDGTFRRIKLRGRQPSCPLCGTQPSITQLIDYEQFCGARADDKDKCLKVLDPAHRITVHEYNKMLETGSNHILVDVRTEVEMDICRLPHQPINIPINRIGQEESIATIQRRLTISEEKPSVVVVCRRGNDSQIAVETLLKCFKDSDLDIKDIRGGLHAWAKHIDTNFPVY

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NEDP02005416
EMBL· GenBank· DDBJ
OWF41195.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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