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A0A1Z1M4N4 · A0A1Z1M4N4_OSMFI

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site52L-glutamine (UniProtKB | ChEBI)
Binding site248L-glutamine (UniProtKB | ChEBI)
Binding site250L-glutamine (UniProtKB | ChEBI)
Active site277Nucleophile
Binding site278L-glutamine (UniProtKB | ChEBI)
Binding site281L-glutamine (UniProtKB | ChEBI)
Binding site317L-glutamine (UniProtKB | ChEBI)
Binding site319L-glutamine (UniProtKB | ChEBI)
Binding site320L-glutamine (UniProtKB | ChEBI)
Active site364
Active site366

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Cellular Componentchloroplast
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA

Encoded on

  • Chloroplast

Organism names

Accessions

  • Primary accession
    A0A1Z1M4N4

Subcellular Location

Keywords

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-176CPSase
Domain8-138Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    391
  • Mass (Da)
    43,914
  • Last updated
    2017-09-27 v1
  • MD5 Checksum
    32AD82A6FC37F67010CDA163E365DC78
MLIPNSVYPSILSLQDGTFYKGWSFFNLSNHFGEIVFNTGMTGYQEILTDPSYSGQLVIFTYPELGNTGLNKEDNESNFIHVKGLVARNISAFCSSWRSNISLKDYLILKRIPHIFGIDTRALTQHIRSFGVMNANIVSYNAYSDYHDIAYQSLDKLDLVKKVTSRNVYKIQAYNHMVTNNSLSIAFNFNNNLIFKKLYTIVIIDFGLKFSIIRKLLLIGCEIYVLPATASYDLILKYKPDGILLSNGPGNPLILTSVINTIKKILYWGNVPIFGICMGHQVLNLALGAKTFKLKFGHRGLNHPSGLNSYAEVTSQNHGFAVARDSLMGNSLLDLLEISQLNLNDLTVASTLHKINPIISVQYHPEASPGPHDSEYLFQVFIKLIELLKCK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MF101415
EMBL· GenBank· DDBJ
ARW60734.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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