A0A1Y7VM56 · A0A1Y7VM56_MOUSE

  • Protein
    NAD-dependent protein deacylase sirtuin-5, mitochondrial
  • Gene
    Sirt5
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site58-77NAD+ (UniProtKB | ChEBI)
Binding site102substrate
Binding site105substrate
Binding site140-143NAD+ (UniProtKB | ChEBI)
Active site158Proton acceptor
Binding site166Zn2+ (UniProtKB | ChEBI)
Binding site169Zn2+ (UniProtKB | ChEBI)
Binding site207Zn2+ (UniProtKB | ChEBI)
Binding site212Zn2+ (UniProtKB | ChEBI)
Binding site249-251NAD+ (UniProtKB | ChEBI)
Binding site275-277NAD+ (UniProtKB | ChEBI)
Binding site296NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrial intermembrane space
Cellular Componentmitochondrial matrix
Cellular Componentnucleus
Molecular FunctionNAD+ binding
Molecular FunctionNAD-dependent protein lysine deacetylase activity
Molecular Functionprotein-glutaryllysine deglutarylase activity
Molecular Functionprotein-malonyllysine demalonylase activity
Molecular Functionprotein-succinyllysine desuccinylase activity
Molecular Functiontransferase activity
Molecular Functionzinc ion binding
Biological Processnegative regulation of reactive oxygen species metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NAD-dependent protein deacylase sirtuin-5, mitochondrial
  • EC number
  • Alternative names
    • Regulatory protein SIR2 homolog 5
    • SIR2-like protein 5

Gene names

    • Name
      Sirt5
    • Synonyms
      SIRT5

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    A0A1Y7VM56

Proteomes

Organism-specific databases

Subcellular Location

Mitochondrion
Cytoplasm, cytosol
Nucleus
Note: Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.

Keywords

PTM/Processing

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Monomer. Homodimer. Interacts with CPS1.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain33-313Deacetylase sirtuin-type

Domain

In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity.

Sequence similarities

Belongs to the sirtuin family. Class III subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    350
  • Mass (Da)
    38,230
  • Last updated
    2017-08-30 v1
  • Checksum
    B2048CF9D70044F4
MRPLLIAPGRFISQLCCRRKPPASPQSKICLTMARPSSNMADFRKCFANAKHIAIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPQAFARNPSQVWEFYHYRREVMRSKEPNPGHLAIAQCEARLRDQGRRVVVITQNIDELHRKAGTKNLLEIHGTLFKTRCTSCGTVAENYRSPICPALAGKGAPEPETQDARIPVDKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELALCDLCLVVGTSSVVYPAAMFAPQVASRGVPVAEFNMETTPATDRFRVSLCSPGCPGTHFVDQAGLELRNLPASASQVLGLKACTTTPGDCTVLISVEDQRGECGQKRESW

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
Q8K2C6SIR5_MOUSESirt5310
A0A1Y7VNW0A0A1Y7VNW0_MOUSESirt595
A0A1Y7VMJ7A0A1Y7VMJ7_MOUSESirt579
A0A1Y7VK33A0A1Y7VK33_MOUSESirt5135
A0A1Y7VJY8A0A1Y7VJY8_MOUSESirt5289

Keywords

Sequence databases

Genome annotation databases

Similar Proteins

Disclaimer

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