A0A1Y2T6V3 · A0A1Y2T6V3_SYMTR

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site43substrate
Binding site81substrate
Binding site103[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site131substrate
Active site133Proton donor
Binding site171substrate
Binding site199[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site227-229substrate
Binding site272substrate

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      A6D92_01000

Organism names

Accessions

  • Primary accession
    A0A1Y2T6V3

Proteomes

Family & Domains

Sequence similarities

Belongs to the IspH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    295
  • Mass (Da)
    32,616
  • Last updated
    2017-08-30 v1
  • Checksum
    D7B33567AA1F3B8A
MEVIKITPRGYCHGVVGAIQLVRRVARDPNVPRPIYVLGQIVHNRHVVEEMEALGVITLDGEDRLSLLEQIDSGTVIFTAHGVSPAVKIRAREKGLHVVDATCPDVTRTHELIAELVGRGYEVIYIGKKGHPEPEGAVGVAPGHVHLVERAEDLDALSFRPDQKLAVTNQTTLSQWDTQALMAEVKARWPQTEVYNEICLATQQRQEAVARMAPEADLVIVVGDRRSNNSNRLVQVAREVAGREAYLVDSVEEIDPAWLKGKRRVAVTSGASTPTHITRAVIEFLEKYDVVVEEL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LWLV01000047
EMBL· GenBank· DDBJ
OTA42181.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp