A0A1Y2SU47 · A0A1Y2SU47_9GAMM

  • Protein
    Carbamoyl phosphate synthase small chain
  • Gene
    carA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site47L-glutamine (UniProtKB | ChEBI)
Binding site241L-glutamine (UniProtKB | ChEBI)
Binding site243L-glutamine (UniProtKB | ChEBI)
Active site269Nucleophile
Binding site270L-glutamine (UniProtKB | ChEBI)
Binding site273L-glutamine (UniProtKB | ChEBI)
Binding site311L-glutamine (UniProtKB | ChEBI)
Binding site313L-glutamine (UniProtKB | ChEBI)
Binding site314L-glutamine (UniProtKB | ChEBI)
Active site353
Active site355

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Molecular Functiontransferase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      Xbed_00688

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • DSM 4764
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Morganellaceae > Xenorhabdus

Accessions

  • Primary accession
    A0A1Y2SU47

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-192CPSase
Domain3-133Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    386
  • Mass (Da)
    42,031
  • Last updated
    2017-08-30 v1
  • Checksum
    0A0E89E0E192DF81
MIKSAILVLEDGTQFHGRAIGAEGVAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGINAADEESPTIQAQGLVIRDLPLVTSNFRSEENLSDYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGNEADAQIAQEKAKAFPGLKGMDLAKEVTTAQSYQWVQGSWTLAGGLPAARQEHELPYHIVAYDFGVKRNILRMLVDRGCRVTVVPAKTSADEVLKMAPDGIFLSNGPGDPEPCDYAIDAIKTFLNTDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDRDVVMITAQNHGFAVDESSLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHEAASLFDHFIELIEQHRQTNVQNSK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MUBK01000003
EMBL· GenBank· DDBJ
OTA21457.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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