A0A1Y1JYW8 · A0A1Y1JYW8_PHOPY

Function

function

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.

Miscellaneous

Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site120Mn2+ 1 (UniProtKB | ChEBI)
Binding site123Mn2+ 1 (UniProtKB | ChEBI)
Binding site123Mn2+ 2 (UniProtKB | ChEBI)
Binding site227-231GMP (UniProtKB | ChEBI)
Binding site228Mn2+ 1 (UniProtKB | ChEBI)
Binding site260Mn2+ 2 (UniProtKB | ChEBI)
Binding site354Mn2+ 2 (UniProtKB | ChEBI)
Binding site354-355GMP (UniProtKB | ChEBI)
Binding site403-406GMP (UniProtKB | ChEBI)
Binding site410GMP (UniProtKB | ChEBI)
Active site429GMP-histidine intermediate
Binding site429-432GMP (UniProtKB | ChEBI)
Binding site505GMP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular ComponenttRNA-splicing ligase complex
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Molecular FunctionRNA ligase (GTP) activity
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-splicing ligase RtcB homolog
  • EC number
  • Alternative names
    • 3'-phosphate/5'-hydroxy nucleic acid ligase

Gene names

    • ORF names
      PPYR_11412
      , PPYR_12172

Organism names

Accessions

  • Primary accession
    A0A1Y1JYW8

Proteomes

Subcellular Location

Interaction

Subunit

Catalytic component of the tRNA-splicing ligase complex.

Family & Domains

Sequence similarities

Belongs to the RtcB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    506
  • Mass (Da)
    55,665
  • Last updated
    2017-08-30 v1
  • Checksum
    2886B40A95B9FEBE
MVVRSYNDELKYLEKINDYCWRIKIGFQPNMKVEGCFYVNAALEKLMLDELRNACRPGMAGGFLPGVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNMAAFDMNDPQSIVSPGGVGFDINCGVRLLRTNLWEKDVLPVKEQLAQSLFDHIPVGVGSKGIIPMNARDLEEALEMGMDWSLREGYVWAEDKEHCEEYGRMLNADPATVSMRAKKRGLPQLGTLGAGNHYAEIQVVDEIYDKWAAGKMGIEEKGQVCVMIHSGSRGFGHQVATDALVQMEKAMKRDNIETNDRQLACAHIHSEEGQNYLKSMAAAANFAWVNRSSMTFLSRQAFAKQFQMSPDDLDMHVIYDVSHNIAKVEEHLVDGKPKTLLVHRKGSTRAFPPGHPLIPVDYQLTGQPVLIGGTMGTCSYVLTGTESGMEQTFGSTCHGAGRALSRAKSRRNLDYTEVLRKLDDMGISIRVASPKLVMEEAPESYKNVTDVVNTCHAAGISNKCIKLRPIAVIKG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GEZM01102990
EMBL· GenBank· DDBJ
JAV51637.1
EMBL· GenBank· DDBJ
Transcribed RNA
VVIM01000008
EMBL· GenBank· DDBJ
KAB0794573.1
EMBL· GenBank· DDBJ
Genomic DNA
VVIM01000008
EMBL· GenBank· DDBJ
KAB0795333.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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