A0A1Y1JGR1 · A0A1Y1JGR1_PLAGO

  • Protein
    Flap endonuclease 1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleolus
Cellular Componentnucleoplasm
Molecular Function5'-3' exonuclease activity
Molecular Function5'-flap endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological Processbase-excision repair
Biological ProcessDNA replication, removal of RNA primer

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flap endonuclease 1
  • EC number
  • Short names
    FEN-1
  • Alternative names
    • Flap structure-specific endonuclease 1

Gene names

    • ORF names
      PGO_030640

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ATCC 30045
  • Taxonomic lineage
    Eukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Haemosporida > Plasmodiidae > Plasmodium > Plasmodium (Plasmodium)

Accessions

  • Primary accession
    A0A1Y1JGR1

Proteomes

Subcellular Location

Nucleus, nucleolus
Nucleus, nucleoplasm
Mitochondrion
Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, coiled coil, compositional bias, region.

TypeIDPosition(s)Description
Domain1-109XPG N-terminal
Domain28-3275'-3' exonuclease
Coiled coil99-128
Domain148-233XPG-I
Compositional bias373-436Basic and acidic residues
Region373-484Disordered
Compositional bias437-454Acidic residues
Region523-544Disordered

Sequence similarities

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    621
  • Mass (Da)
    70,652
  • Last updated
    2017-08-30 v1
  • Checksum
    57E728FCE4FEDF6B
MGIKGLTKFIADAAPNAIKEIKIENLMGRVVAIDASMSLYQFIIAIRDSEQYGNLTNELGETTSHISGLMSRSIKLMENGLKPIYVFDGAPPELKGSELEKRGEKRQKAEELLKKAKEEGNIEEIKKQSGRTVRVTKKQNEEAKKLLTLMGIPVVEAPCEAESQCAFLTKYNLAHATATEDADALVFGTKILIRNLNANASSSANQNKNKNSSKRGYILTEINLEQVLKGLNLNMNEFIDFCILCGCDYCDTIKGIGSKTAYNLIKEYNCIEKIIENIDKNKYQVPANFRFVEARDSFINPKVLSKEEVKIDWCEPKIEELKNFLIKDYNFNEVRVTNYINRLLKARKATTQRRLDNFFTACTKKSTKLIIEESQKELKPKRKGKKRDLPIDGSTKLNAKQKKEQNKKVKVENEDKKTSIKNESKEELQNHHMEEAEEGEVEEEVEEEVEEEKDDAMGLAPPFDEEQDEGDNPSPNFFHHKSDSESGNLKIAEMEKMEEIEEMEQNVKNDNISPVYNCTNGNDTLGNNTHLSSNSTSYSRNSLNNNKKIDESVKHLTNDSPRDDNNHLCSSELFPHNTTDCLNNNSDMMQSQVKKKNILFLLPYCPKNVTSVKKRKSIQRF

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias373-436Basic and acidic residues
Compositional bias437-454Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BDQF01000003
EMBL· GenBank· DDBJ
GAW79264.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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