A0A1Y0T1Y6 · A0A1Y0T1Y6_MYCIT
- ProteinLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT
- GenemurT
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids409 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.
Catalytic activity
- ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H+ + L-glutamate + phosphate
- ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate
- beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4+ = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H+ + phosphate
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 333 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acid-amino acid ligase activity | |
Molecular Function | ATP binding | |
Molecular Function | carbon-nitrogen ligase activity on lipid II | |
Molecular Function | metal ion binding | |
Biological Process | cell wall organization | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium avium complex (MAC)
Accessions
- Primary accessionA0A1Y0T1Y6
Proteomes
Interaction
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length409
- Mass (Da)43,092
- Last updated2017-10-25 v1
- Checksum6DD0AED13060074F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP015267 EMBL· GenBank· DDBJ | ASL12824.1 EMBL· GenBank· DDBJ | Genomic DNA |