A0A1Y0SWV5 · A0A1Y0SWV5_MYCIT
- ProteinLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
- GenecobQ2
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids235 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT.
Catalytic activity
- ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H+ + L-glutamate + phosphate
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 94 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 128 | substrate | ||||
Sequence: R | ||||||
Active site | 194 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbon-nitrogen ligase activity on lipid II | |
Molecular Function | glutaminase activity | |
Biological Process | cell wall organization | |
Biological Process | cobalamin biosynthetic process | |
Biological Process | glutamine metabolic process | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium avium complex (MAC)
Accessions
- Primary accessionA0A1Y0SWV5
Proteomes
Interaction
Structure
Sequence
- Sequence statusComplete
- Length235
- Mass (Da)24,602
- Last updated2017-10-25 v1
- ChecksumF86995B197ED46FF