A0A1Y0N2T7 · A0A1Y0N2T7_9BURK

  • Protein
    2-isopropylmalate synthase
  • Gene
    leuA_1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site14Mn2+ (UniProtKB | ChEBI)
Binding site202Mn2+ (UniProtKB | ChEBI)
Binding site204Mn2+ (UniProtKB | ChEBI)
Binding site239Mn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function2-isopropylmalate synthase activity
Molecular Functionacetyl-CoA C-acetyltransferase activity
Molecular Functionmanganese ion binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-isopropylmalate synthase
  • EC number
  • Alternative names
    • Alpha-IPM synthase
    • Alpha-isopropylmalate synthase

Gene names

    • Name
      leuA_1
    • Synonyms
      leuA
    • ORF names
      AEP_00019

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • AEP1-3
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Curvibacter

Accessions

  • Primary accession
    A0A1Y0N2T7

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain5-268Pyruvate carboxyltransferase
Region394-512Regulatory domain

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    512
  • Mass (Da)
    55,162
  • Last updated
    2017-08-30 v1
  • MD5 Checksum
    B3E9BF7F306F92ADED85DAF5249D55E7
MTDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLRVDVIEAGFAASSNGDFEAVQLIANSIKDSTVCSLSRANDRDIARAAEALKGANSARIHTFIATSALHMEKKLRMTPEQVLEQAKQSVRFARNLVADIEFSPEDGYRSDPDFLCRVLEAVIAEGASTINVPDTVGYAVPELYGNFIKNLRERIPNSDKAIWSVHCHNDLGMAVANSLAGVKIGGARQVECTVNGLGERAGNCSLEEIVMAVKTRKDYFGLELGIDAKHILAASRMVSQTTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRVEDVGWTANKLVLGKLSGRNAFKQRLQELGVQLDSESEINAAFARFKELADRKSEIFDEDILALVGDESVSLESEQYGFVSLSQHSETGERPRAAIVFTAGGKEMKGESDGNGPVDASLKAIESLVKSGAEMVLYSVNAISGSTESQGEVTVRLQNSGRVVNGVGADPDIVVASAKAYLSALNKLQNKADRVAAQG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP015698
EMBL· GenBank· DDBJ
ARV16987.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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