A0A1Y0N2T7 · A0A1Y0N2T7_9BURK
- Protein2-isopropylmalate synthase
- GeneleuA_1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids512 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
Catalytic activity
- 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H+
Cofactor
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 14 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 202 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 204 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 239 | Mn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 2-isopropylmalate synthase activity | |
Molecular Function | acetyl-CoA C-acetyltransferase activity | |
Molecular Function | manganese ion binding | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-isopropylmalate synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Curvibacter
Accessions
- Primary accessionA0A1Y0N2T7
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 5-268 | Pyruvate carboxyltransferase | |||
Region | 394-512 | Regulatory domain | |||
Sequence similarities
Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length512
- Mass (Da)55,162
- Last updated2017-08-30 v1
- MD5 ChecksumB3E9BF7F306F92ADED85DAF5249D55E7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP015698 EMBL· GenBank· DDBJ | ARV16987.1 EMBL· GenBank· DDBJ | Genomic DNA |