A0A1X9Y2S9 · A0A1X9Y2S9_9GAMC

Function

function

Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.
Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.
Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Alone is able to induce paired membranes. Coexpression of nsp3 and nsp4 does not result in the formation of DMVs.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.
Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site5559
Active site5561
Active site5660
Active site5736
Active site5741
Binding site5799-5805S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6214
Active site6229
Active site6269

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Gene names

    • Name
      1ab

Organism names

  • Taxonomic identifier
  • Strain
    • SNU-10043
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Gammacoronavirus > Igacovirus > Avian coronavirus

Accessions

  • Primary accession
    A0A1X9Y2S9

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1727-1746Helical
Transmembrane1753-1775Helical
Transmembrane1846-1873Helical
Transmembrane1885-1910Helical
Transmembrane2283-2301Helical
Transmembrane2560-2582Helical
Transmembrane2588-2609Helical
Transmembrane2616-2633Helical
Transmembrane2639-2667Helical
Transmembrane3099-3119Helical
Transmembrane3125-3142Helical
Transmembrane3154-3177Helical
Transmembrane3197-3214Helical
Transmembrane3226-3249Helical
Transmembrane3269-3287Helical
Transmembrane3299-3322Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1804↔1810

Keywords

Interaction

Subunit

Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Interacts with ORF6 protein.
Homodimer.
Interacts with host PHB and PHB2.
Interacts with papain-like protease and non-structural protein 6.
Monomer. Homodimer. Only the homodimer shows catalytic activity.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain675-780Ubiquitin-like
Domain1005-1181Macro
Domain1177-1229Ubiquitin-like
Domain1238-1499Peptidase C16
Domain1771-18353Ecto
Region1913-2003Y1
Domain1913-2265CoV Nsp3 Y
Region1917-1930ZF1
Region1963-1973ZF2
Region2004-2265CoV-Y
Region2166-2265Y4
Domain2686-2781Nsp4C
Domain2782-3088Peptidase C30
Domain3382-3464RdRp Nsp7 cofactor
Domain3465-3674RdRp Nsp8 cofactor
Domain3675-3785Nsp9 ssRNA-binding
Domain3787-3928ExoN/MTase coactivator
Domain3942-4200NiRAN
Domain4205-4303Nsp12 Interface
Domain4304-4870Nsp12 RNA-dependent RNA polymerase
Domain4550-4712RdRp catalytic
Domain4871-4954CV ZBD
Domain5127-5479+RNA virus helicase C-terminal
Domain5541-5755ExoN
Domain5764-5991N7-MTase
Region5879-5893GpppA-binding
Domain5992-6052Nsp15 N-terminal oligomerization
Domain6053-6168AV-Nsp11N/CoV-Nsp15M
Domain6185-6326NendoU
Domain6329-6628Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,631
  • Mass (Da)
    744,175
  • Last updated
    2017-08-30 v1
  • MD5 Checksum
    959BEC4ED620C73D006C4AC1CC4BDF07

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KU900743
EMBL· GenBank· DDBJ
ARS22458.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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