A0A1X0YCW1 · A0A1X0YCW1_9BACT
- Proteinhistidine kinase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids742 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.
Catalytic activity
- N6-(pyridoxal phosphate)-L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + L-histidyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 2 Fe3+ + 4 H2O = L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + (2S)-2-amino-5-hydroxy-4-oxopentanoyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 3-oxopropanoate + 2 Fe2+ + 2 H+This reaction proceeds in the forward direction.
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | metal ion binding | |
Molecular Function | phosphorelay sensor kinase activity | |
Biological Process | thiamine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namehistidine kinase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfuromonadia > Desulfuromonadales > Geothermobacteraceae > Geothermobacter
Accessions
- Primary accessionA0A1X0YCW1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 331-351 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-27 | ||||
Chain | PRO_5013072223 | 28-742 | histidine kinase | ||
Keywords
- PTM
Interaction
Structure
Family & Domains
Features
Showing features for coiled coil, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Coiled coil | 364-391 | ||||
Domain | 526-742 | Histidine kinase | |||
Sequence similarities
Belongs to the NMT1/THI5 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length742
- Mass (Da)83,007
- Last updated2017-07-05 v1
- MD5 ChecksumBDFC27A29D7779064156624AB5162927
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
NAAD01000002 EMBL· GenBank· DDBJ | ORJ62923.1 EMBL· GenBank· DDBJ | Genomic DNA |