A0A1X0NTC6 · A0A1X0NTC6_9TRYP
- ProteinRNA cytidine acetyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1061 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N4-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires a tRNA-binding adapter protein for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.
Catalytic activity
- a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = an N4-acetylcytidine in 18S rRNA + ADP + phosphate + CoA + H+
- a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N4-acetylcytidine in tRNA + ADP + phosphate + CoA + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 318-327 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GRGKSAALGL | ||||||
Binding site | 499 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 658-660 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: IAT | ||||||
Binding site | 665-671 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: QRAGYGS | ||||||
Binding site | 761 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | 90S preribosome | |
Cellular Component | nucleolus | |
Molecular Function | ATP binding | |
Molecular Function | rRNA cytidine N-acetyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA N-acetyltransferase activity | |
Biological Process | rRNA acetylation involved in maturation of SSU-rRNA | |
Biological Process | tRNA acetylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA cytidine acetyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma
Accessions
- Primary accessionA0A1X0NTC6
Proteomes
Organism-specific databases
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-24 | Disordered | ||||
Sequence: MSSSASVVSTAHSARGGSGGGVTI | ||||||
Domain | 30-224 | tRNA(Met) cytidine acetyltransferase TmcA N-terminal | ||||
Sequence: DRIRNLIENAVHKKHRALILLVGDRGKDQIVNLHQMVSHANHNAKVNMLWCMKKDPDFGSTSKKQQEKRARLEVKGGMSTETTKEAFQTFLSQTQIRFCQYKETHKVLGQTFGMAVLQDFEAITPNTLARTIETVKGGGVVVIMFRAMRSLKQLYTIAMDVHSRYRTEALRDVVPRFNERFLLSLVDCDTALCVD | ||||||
Domain | 312-517 | Helicase | ||||
Sequence: VVTAGRGRGKSAALGLAAAGALAQGYSNIFCTAPSPENLQTFFEFVVRGLQEQGYKERTDFEAMQSTNVEFSKCIVRINLFREHRQTVQYISPHDADKFAQAELAIIDEAAALPLPLVKQMLGPYLVFLSSTVSGYEGTGRSLSMKLIADMRKQSGSIVGGSSSTSTAVSTSDDRRFLREITMTDPIRYGPNDPVESWLHKLLCLD | ||||||
Domain | 558-788 | N-acetyltransferase | ||||
Sequence: VVAMLVAAHYKNQPNDLQLMSDAPGHHLFLLCSSTVETNGELPDVFCVIHACEEGQVTSESIKSNLSRGVRPSGDLIPYTLSQYYLEEGFAKLAGLRIVRIATNPELQRAGYGSRALELLQQYYTGSISLQPASSESLKTKEETENNNNDNDDNDDTNSTSNVITPRKRIPSLLTSLVERPYEVIDYLGVSFGVTTPLFNFWKRAGYEALYLRHAANELTGEHSCVMVRPI | ||||||
Region | 691-721 | Disordered | ||||
Sequence: SSESLKTKEETENNNNDNDDNDDTNSTSNVI | ||||||
Domain | 797-997 | Possible tRNA binding | ||||
Sequence: AEFHQRFIPLLSMPFRDLPTELALSILNDLDVNDPQKLAAATDHSQSSEHIVRVGGRLQCTLRELQLIFNQNDMKRLRLNATTFVEGGLVLDLLPALAKLYFEARLHRLPDGSEGVVLTHAQAAVLLAVGLQCQTIEELSRQSAFAGVPLQQLRAFLQKAVARIVEHFTRLEKLKTPQEANGHSSVKNGEEGEEEDGEQEQ | ||||||
Region | 971-1001 | Disordered | ||||
Sequence: KTPQEANGHSSVKNGEEGEEEDGEQEQTREI | ||||||
Region | 1034-1061 | Disordered | ||||
Sequence: AAIGTSKSESGTTGIQRAFKRSKKTRRS | ||||||
Compositional bias | 1036-1051 | Polar residues | ||||
Sequence: IGTSKSESGTTGIQRA |
Sequence similarities
Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,061
- Mass (Da)117,670
- Last updated2017-07-05 v1
- Checksum0124E3A7B9DB300C
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1036-1051 | Polar residues | ||||
Sequence: IGTSKSESGTTGIQRA |
Keywords
- Technical term