A0A1W5YIR2 · A0A1W5YIR2_ACTAR
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids685 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 76 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 78 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 94 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 97 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 496 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 498 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 500 | Mg2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > Ericales > Actinidiaceae > Actinidia
Accessions
- Primary accessionA0A1W5YIR2
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Sequence
- Sequence statusComplete
- Length685
- Mass (Da)78,923
- Last updated2017-07-05 v1
- ChecksumC2673BEC25429762
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KY100978 EMBL· GenBank· DDBJ | ARI43521.1 EMBL· GenBank· DDBJ | Genomic DNA |