A0A1W5XWI5 · A0A1W5XWI5_9ACTN

  • Protein
    Lysine--tRNA ligase
  • Gene
    lysS
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ ions per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site1017Mg2+ 1 (UniProtKB | ChEBI)
Binding site1024Mg2+ 1 (UniProtKB | ChEBI)
Binding site1024Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionlysine-tRNA ligase activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleic acid binding
Molecular Functionphosphatidylglycerol lysyltransferase activity
Biological Processlipid metabolic process
Biological Processlysyl-tRNA aminoacylation
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lysine--tRNA ligase
  • EC number
  • Alternative names
    • Lysyl-tRNA synthetase
      (LysRS
      )

Gene names

    • Name
      lysS
    • ORF names
      STRMOE7_32555

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • MOE7
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A1W5XWI5

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane24-45Helical
Transmembrane51-78Helical
Transmembrane85-106Helical
Transmembrane112-129Helical
Transmembrane141-164Helical

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain790-1098Aminoacyl-transfer RNA synthetases class-II family profile

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the LPG synthetase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,105
  • Mass (Da)
    121,659
  • Last updated
    2017-07-05 v1
  • Checksum
    345E78910B4AF9FA
MSTVQDQDQLTGWRRFRRRVPNGFAIVFSVLGLFCALTALIGPLRRGLHPVIYWLDTLTIPVAPNFAYAAFLFLLGAAMAARKRVALWFVVAYMVLVTLADALFLAAGYWEFAFSLVLCAGALALLLVSHHEFYAITRRGAFLRAIGVLIGGLVAAVLIGWGLVTLAPGTLEAGGGNRLLWAANRVCGGLVGGHLVEGRPPHWTSTVLGLLGALALLNAAAALFRSQRMEAALHGDEEDRIRALLERYGSQDSLGYFASRRDKAVVFSPSGKAAVTYRVEAGVCLASGDPVGDREAWTQAIEAWLDVAGRYGWQPAVMGASESGAKAFARCGLGALQLGDEAILHVKDFDLDGREMRVTRQAVNRVERAGATFRVRRHSALTDEEMQEVIHRADAWRDTETERGFSMALDRLGDPADGDCMLAEAFDGDGNMIALLSFVPWGKDGISLDVMRRDRTAPNGVMEFMVAQLCAQAGSLGVRRISLNFAVFRSAFEEGARIGAGPVLKFWRRLLLFFSKWWQLEALYRSNAKYNPEWYPRFLCYADAGALARIGLASGIAEGFVAVPSLGKLWGKGHKKRVLAPASTAGLPSLDELGLVQAAPATEEELHEQELAALPEQVRVRHRKLERLREAGTDPYPVGVQRTHTLGEVREEYPDLTPGARTGKSVSVAGRVLLTRDHGGVLFAVLRDWSGDLQIALTRDGSGKDLLDRFGSDIDLGDHVEAEGEIGASDRGELTVFVTKWRLTAKCLRPLPDKRRGLSDPEAKVRQRYVDLVVSTDARENVRARSTAVQALRQGLIDRGYLEVETPMLQQIHGGANARPFHTHINAYDLDLYLRIAPELYLKRLCVGGMEKVFEMGRTFRNEGISYKHNPEFTMLEAYQAFADYDVMLDLTRELIQGAAIAAFGSATARKADANGRLVEHDISGIWPVKTVYGAISEALGEEVDADTEADRLRRLCNAASVPVKPEMGRGDIVLEMYERLVEEKTQLPTFYKDFPTDVSPLTRQHRRDPRLAERWDLVAFGTELGTAYSELTDPVEQRRRLTAQSLLAAGGDPEAMELDEDFLQALEYAMPPTGGLGIGVDRLVMFLTGLSIRETLPFPLVRRR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP019779
EMBL· GenBank· DDBJ
ARH94240.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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