A0A1W2EWT1 · A0A1W2EWT1_9FIRM

  • Protein
    ATP-dependent 6-phosphofructokinase
  • Gene
    pfkA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site17ATP (UniProtKB | ChEBI)
Binding site79-80ATP (UniProtKB | ChEBI)
Binding site119-122ATP (UniProtKB | ChEBI)
Binding site120Mg2+ (UniProtKB | ChEBI); catalytic
Site121Important for substrate specificity; cannot use PPi as phosphoryl donor
Binding site142-144substrate; ligand shared between dimeric partners; in other chain
Active site144Proton acceptor
Binding site179substrate; ligand shared between dimeric partners
Binding site186-188substrate; ligand shared between dimeric partners; in other chain
Binding site239substrate; ligand shared between dimeric partners; in other chain
Binding site283substrate; ligand shared between dimeric partners
Binding site289-292substrate; ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Component6-phosphofructokinase complex
Molecular Function6-phosphofructokinase activity
Molecular FunctionAMP binding
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionfructose-6-phosphate binding
Molecular Functionidentical protein binding
Molecular Functionmetal ion binding
Molecular Functionmonosaccharide binding
Biological Processcanonical glycolysis
Biological Processfructose 1,6-bisphosphate metabolic process
Biological Processfructose 6-phosphate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent 6-phosphofructokinase
  • EC number
  • Short names
    ATP-PFK
    ; Phosphofructokinase
  • Alternative names
    • Phosphohexokinase

Gene names

    • Name
      pfkA
    • ORF names
      SAMN04488500_1339

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • DSM 5090
  • Taxonomic lineage
    Bacteria > Bacillota > Negativicutes > Selenomonadales > Sporomusaceae > Sporomusa

Accessions

  • Primary accession
    A0A1W2EWT1

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer or homotetramer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain10-315Phosphofructokinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    365
  • Mass (Da)
    38,751
  • Last updated
    2017-06-07 v1
  • Checksum
    6C241EEBE3B1BEB5
MANVNKVKTIAVLTGGGDAPGLNAVIRAVVRTALGQGLKVWGIKNGFGGMVENQMIEMTDSSVAGILPRGGTILGTTNRDNPFHYPKKQDSQIVYENMSAQALCNLRERDIEAIVVIGGDGSLKIASEFYHLGLPVVAVPKTIDNDIPGTERTFGFDTAVGVATDALDRLHTTAESHHRVMVLEVMGRYAGWIALHAGMAGGADCILLPEIPFAIDSVVAKIKEREARGRLFSLVVIAEGAYPQGGEQSVSLIVEGSHEKVRLGGAGEKLAREIEKLTGVESRCTVLGHVQRGGTPTAFDRVLSTRYGVAAAKCIFDGAFGNMVALRKNQIIRVPIDEIAGKAHNVTADDELLQAGRAIGICFGD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FWXI01000033
EMBL· GenBank· DDBJ
SMD14154.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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