A0A1W1UGD4 · A0A1W1UGD4_DESTI

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site35-39NAD+ (UniProtKB | ChEBI)
Binding site84-85NAD+ (UniProtKB | ChEBI)
Binding site115NAD+ (UniProtKB | ChEBI)
Active site117N6-AMP-lysine intermediate
Binding site138NAD+ (UniProtKB | ChEBI)
Binding site173NAD+ (UniProtKB | ChEBI)
Binding site288NAD+ (UniProtKB | ChEBI)
Binding site312NAD+ (UniProtKB | ChEBI)
Binding site406Zn2+ (UniProtKB | ChEBI)
Binding site409Zn2+ (UniProtKB | ChEBI)
Binding site429Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological Processbase-excision repair, DNA ligation
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      SAMN00017405_0842

Organism names

Accessions

  • Primary accession
    A0A1W1UGD4

Proteomes

Subcellular Location

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain590-666BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    666
  • Mass (Da)
    74,593
  • Last updated
    2017-06-07 v1
  • Checksum
    8FD8F041B251C18D
MLTLELAKEKINTLKEQINDHNYSYYVLDKPIVSDDVYDNLMNELLNLEKEFPELITSDSPSMRVGGQPLDTFKTYKHRSPLLSLGNSFNKNDLIEFDKRIVNSLGEAEVNYVVEPKIDGLSIALYYKNGVLEIASTRGDGLVGENVTQNVKTIPNIPLKLKDNMPVLEVRGEVYMPKSSFERLNMERQSNNQELFANPRNAAAGSIRQLDPKVAASRDLRAFIYTVMYVDGKKIENHIEALEFLKEQGFSTTEIFVSNDINEVYDYCTKWNIKRKDLPYEIDGMVIKVNSLKVQEKLGYTSKSPRWAIAYKFPAEQVETKIIDITVRVGRTGAVTPTAHLEPVKIAGTIVSRATLHNEDFVKEKNIKIGDYVVIQKAGDIIPEVVEVINEKRTGNEVDFELPVVCPECGSKIIRPTGEAIARCSGLSCPAQIREGIIHFVSRNAMDIDGLGPAVINQLLENKLISDASDLYYLQFEDLIKLERMGEKSAQNLLQAIEKSKDNSLGQLIFALGIRLVGQRAGKILADHYRSLEKLKNTSLDELMTIPEIGPKMAESVVQFFKDEKNVIFIERLKEAKITTENSSPIEVTTISSKLSGKTFVVTGSLEKFSRKDIKDKIESLGAKVSSSVSKKTDFVLVGKDPGSKYDKAVEIGITILTEDEFLAMI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FWWT01000005
EMBL· GenBank· DDBJ
SMB80165.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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