A0A1V3B6J8 · A0A1V3B6J8_HELPX

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site89Proton acceptor
Binding site123[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site127[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site130[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site173-174S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site203S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site226-228S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site302S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site345S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • ORF names
      BZK22_06925
      , C2R54_07825
      , F7195_06720

Organism names

  • Taxonomic identifier
  • Strains
    • SV397_2
    • B41
    • MHP47
  • Taxonomic lineage
    Bacteria > Campylobacterota > Epsilonproteobacteria > Campylobacterales > Helicobacteraceae > Helicobacter

Accessions

  • Primary accession
    A0A1V3B6J8

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain109-340Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    357
  • Mass (Da)
    40,717
  • Last updated
    2017-06-07 v1
  • Checksum
    ABC43815EC204CFF
MKASIYDFTLDELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIAYLEQEFTLRTIEIAHVRESVDGSKKYLFKSLRDNHTFEAVLLKMKDKKIDEETNAVLEGEKYTVCVSCQIGCQVGCSFCFTQKGGFVRDLKASEIIQQALLIKEDNNLPIEKALNIVFMGMGEPLNNLDEVCKAIEIFNTGMQISPKRITISTSGVADKIPILAGKNLGVQLAISLHAVDDKTRSSLMPLNKKYNIECVLNEVRKWPLEQRKRVMFEYLLIKDLNDSLDCAKKLLKLLNGIKSKVNLILFNPHEGSKFERPSLESARMFADFLNAKGLLCTIRESKALDIEAACGQLREKKLQQKI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
WACX01000015
EMBL· GenBank· DDBJ
MUU45816.1
EMBL· GenBank· DDBJ
Genomic DNA
MTWS01000020
EMBL· GenBank· DDBJ
OOC29331.1
EMBL· GenBank· DDBJ
Genomic DNA
QBRK01000063
EMBL· GenBank· DDBJ
PUD58856.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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