A0A1V0E492 · TPS1_PIPNI
- ProteinTerpene synthase 1
- GeneTPS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids559 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Sesquiterpene synthase involved in the biosynthesis of volatile compounds that contribute to the characteristic flavors of black pepper (PubMed:29248443).
Mediates the conversion of (2E,6E)-farnesyl diphosphate (FPP) into beta-caryophyllene and, as a minor compound, into alpha-humulene (PubMed:29248443).
Mediates the conversion of (2E,6E)-farnesyl diphosphate (FPP) into beta-caryophyllene and, as a minor compound, into alpha-humulene (PubMed:29248443).
Catalytic activity
- (2E,6E)-farnesyl diphosphate = --(E)-beta-caryophyllene + diphosphateThis reaction proceeds in the forward direction.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 3 Mg2+ or Mn2+ ions per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
21 μM | (2E,6E)-farnesyl diphosphate |
kcat is 0.455 sec-1 with (2E,6E)-farnesyl diphosphate as substrate.
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 312 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 312 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 316 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 316 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 456 | Mg2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 464 | Mg2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | alpha-humulene synthase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | beta-caryophyllene biosynthetic process | |
Biological Process | diterpenoid biosynthetic process | |
Biological Process | green leaf volatile biosynthetic process | |
Biological Process | sesquiterpene biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTerpene synthase 1
- Short namesPnTPS1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Magnoliidae > Piperales > Piperaceae > Piper
Accessions
- Primary accessionA0A1V0E492
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454951 | 1-559 | Terpene synthase 1 | |||
Sequence: MACVSDLVAFTQPLIIGAKPLEIVRRSAAFHPNVWGDYFLKLSQDEKKLESMRERAKVLKEKVLKKLSTIEGGERLELIDTLYHLGVAYNFEKEIEEALEKIYKAYDEDATQDNLCTLALRFRLLRQHGWNASSDVFNKFKETKNGNFKESVASDVLGMLSLYEASYVGTKEDKILEEAISFTTRNLSAALPNMEPLLAERVAHSLELPLHKRLQRLEARYFITMYEKNNAHDEMLLEYAKLDYNLLQALHQNEMKELTKWWTKIDLVGKMKFPRDRVTECYFWPLGAFFEPQHSRGRIFATKITQLTSIIDDLYDVYGTQEELQLFTDVIQRWDMNAKKSLPDYIKPLYEALLSTLKDFEEELSLEGNAYRASFMQQAMKNICMAYFDEAKWYNRGTTPKVEEYLNSAEISCGYPVVATACFTGAGEITTKKLLEWIQSQPKYMKDTCRLCRIVDDIKTYKFEEERGHVASVVACYMEEHKCNEDEALEKLNEDVMNTWKDINKACMRPTPFPMVVMNIIRNLSRVMEILYQFGDGYTFADTVTKERLNLLLKDPIPV |
Expression
Tissue specificity
Mostly expressed in stems and, to a lower extent, in leaves, roots and fruits.
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 312-316 | DDXXD motif | ||||
Sequence: DDLYD |
Domain
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family. Tpsa subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length559
- Mass (Da)64,936
- Last updated2017-06-07 v1
- Checksum2CCC2217B4782385