A0A1V0E492 · TPS1_PIPNI

Function

function

Sesquiterpene synthase involved in the biosynthesis of volatile compounds that contribute to the characteristic flavors of black pepper (PubMed:29248443).
Mediates the conversion of (2E,6E)-farnesyl diphosphate (FPP) into beta-caryophyllene and, as a minor compound, into alpha-humulene (PubMed:29248443).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 3 Mg2+ or Mn2+ ions per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
21 μM(2E,6E)-farnesyl diphosphate
kcat is 0.455 sec-1 with (2E,6E)-farnesyl diphosphate as substrate.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site312Mg2+ 1 (UniProtKB | ChEBI)
Binding site312Mg2+ 2 (UniProtKB | ChEBI)
Binding site316Mg2+ 1 (UniProtKB | ChEBI)
Binding site316Mg2+ 2 (UniProtKB | ChEBI)
Binding site456Mg2+ 3 (UniProtKB | ChEBI)
Binding site464Mg2+ 3 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionalpha-humulene synthase activity
Molecular Functionmagnesium ion binding
Molecular Functionterpene synthase activity
Biological Processbeta-caryophyllene biosynthetic process
Biological Processditerpenoid biosynthetic process
Biological Processgreen leaf volatile biosynthetic process
Biological Processsesquiterpene biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Terpene synthase 1
  • Short names
    PnTPS1
  • Alternative names
    • Alpha-humulene synthase
      (EC:4.2.3.104
      ) . EC:4.2.3.104 (UniProtKB | ENZYME | Rhea)
    • Beta-caryophyllene synthase
      (PnCPS
      ) (EC:4.2.3.57
      ) . EC:4.2.3.57 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      TPS1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Magnoliidae > Piperales > Piperaceae > Piper

Accessions

  • Primary accession
    A0A1V0E492

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004549511-559Terpene synthase 1

Expression

Tissue specificity

Mostly expressed in stems and, to a lower extent, in leaves, roots and fruits.

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif312-316DDXXD motif

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similarities

Belongs to the terpene synthase family. Tpsa subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    559
  • Mass (Da)
    64,936
  • Last updated
    2017-06-07 v1
  • Checksum
    2CCC2217B4782385
MACVSDLVAFTQPLIIGAKPLEIVRRSAAFHPNVWGDYFLKLSQDEKKLESMRERAKVLKEKVLKKLSTIEGGERLELIDTLYHLGVAYNFEKEIEEALEKIYKAYDEDATQDNLCTLALRFRLLRQHGWNASSDVFNKFKETKNGNFKESVASDVLGMLSLYEASYVGTKEDKILEEAISFTTRNLSAALPNMEPLLAERVAHSLELPLHKRLQRLEARYFITMYEKNNAHDEMLLEYAKLDYNLLQALHQNEMKELTKWWTKIDLVGKMKFPRDRVTECYFWPLGAFFEPQHSRGRIFATKITQLTSIIDDLYDVYGTQEELQLFTDVIQRWDMNAKKSLPDYIKPLYEALLSTLKDFEEELSLEGNAYRASFMQQAMKNICMAYFDEAKWYNRGTTPKVEEYLNSAEISCGYPVVATACFTGAGEITTKKLLEWIQSQPKYMKDTCRLCRIVDDIKTYKFEEERGHVASVVACYMEEHKCNEDEALEKLNEDVMNTWKDINKACMRPTPFPMVVMNIIRNLSRVMEILYQFGDGYTFADTVTKERLNLLLKDPIPV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KU953957
EMBL· GenBank· DDBJ
ARB08605.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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