A0A1T0APK5 · A0A1T0APK5_9PAST
- ProteinEnergy-dependent translational throttle protein EttA
- GeneettA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids556 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. ATP hydrolysis probably frees it from the ribosome, which can enter the elongation phase.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ribosome binding | |
Molecular Function | rRNA binding | |
Molecular Function | tRNA binding | |
Biological Process | negative regulation of translational elongation | |
Biological Process | translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEnergy-dependent translational throttle protein EttA
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pasteurellales > Pasteurellaceae > Haemophilus
Accessions
- Primary accessionA0A1T0APK5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Associates with ribosomes and polysomes.
Keywords
- Cellular component
Interaction
Subunit
Monomer. Probably contacts ribosomal proteins L1, L5, L33 and S7, the 16S and 23S rRNA and the P-site containing tRNA(fMet).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 7-261 | ABC transporter | ||||
Sequence: YTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGAGKSTLLRIMAGIDKEIEGEARPQPGIKIGYLPQEPKLEPQQTVREAIEEAVGEVKKALTRLDEVYALYADPDADFDKLAAEQAELEAIIQAHDGHNLDNQLERAADALRLPDWDAKIEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITHDRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQA | ||||||
Region | 96-140 | Arm | ||||
Sequence: GEVKKALTRLDEVYALYADPDADFDKLAAEQAELEAIIQAHDGHN | ||||||
Region | 243-323 | PtIM | ||||
Sequence: GNYSSWLEQKEKRLAQEQAAESARQKSIEKELEWVRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDK | ||||||
Domain | 325-551 | ABC transporter | ||||
Sequence: IEVQNLTKSYGDRTLIDDLSFSIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASVDQFRDAMDDKKTVWEEVSGGQDIMRIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGERGRLHLAKLLQVGGNVLLLDEPTNDLDVETLRALENAILEFPGCALVISHDRWFLDRIATHILDYGDEGKVTFYEGNFSDYEEWKKKTYGEAATQPHRIKY |
Domain
The P-site tRNA interaction motif (PtIM domain) probably interacts with the P-site tRNA(fMet) as well as the 23S rRNA.
The arm domain is inserted in the first ABC transporter domain. Probably contacts ribosomal protein L1.
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length556
- Mass (Da)62,530
- Last updated2017-05-10 v1
- Checksum488FB88815C1A0F2
Keywords
- Technical term