A0A1S6YI91 · A0A1S6YI91_9EUKA

  • Protein
    Queuine tRNA-ribosyltransferase catalytic subunit 1
  • Gene
    tgt
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site103Proton acceptor
Binding site103-107substrate
Binding site157substrate
Binding site199substrate
Binding site226substrate
Active site276Nucleophile
Binding site314Zn2+ (UniProtKB | ChEBI)
Binding site316Zn2+ (UniProtKB | ChEBI)
Binding site319Zn2+ (UniProtKB | ChEBI)
Binding site345Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplastid
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological ProcesstRNA wobble guanine modification
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase catalytic subunit 1
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • Name
      tgt
    • Synonyms
      MYN1_Chr_444
    • ORF names
      PFK_500
      , PMYN1_Chma453

Encoded on

  • Plastid

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • FK01
    • MYN1
  • Taxonomic lineage
    Eukaryota > Sar > Rhizaria > Cercozoa > Imbricatea > Silicofilosea > Euglyphida > Paulinellidae > Paulinella

Accessions

  • Primary accession
    A0A1S6YI91

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Heterodimer of a catalytic subunit and an accessory subunit.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain25-373tRNA-guanine15 transglycosylase-like
Region257-263RNA binding
Region281-285RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    383
  • Mass (Da)
    42,390
  • Last updated
    2017-05-10 v1
  • MD5 Checksum
    D420CBB2463982C400131B4FF46CCB28
MNVINKSKELSPFKFEIIAECTRTRARCGHFSTPHGLVYTPQFMPVGTTATVKGISPEQLSATKAQIVLANTFHLHLQPGEDIVARAGGLHHFMGWNGPILTDSGGFQVFSLGNINSINDSGVIFQSPRDGSKVELTPEKATRIQMALGADIAMAFDQCPPPSANEEAMRLACERTYLWLKRCISIHDNKNQALFGIVQGGCFLHLREKCVKEITELELHGNAIGGVSVGESVHEIHRIIRQVTPLLPKQKPRYLMGVGTLPEMNIAVANGIDLFDCVLPTRLGRHGTALVKGDRWNLRNARFREDYTILEEGCPCPACKYLSRAYLHHLVRSDELLGVTLLSLHNLTVLIRFTTAMAQAIRDGCFSEDFAPWKNDSPAAHTW

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KY124271
EMBL· GenBank· DDBJ
AQX45049.1
EMBL· GenBank· DDBJ
Genomic DNA
LC490351
EMBL· GenBank· DDBJ
BBL86262.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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