A0A1S5WJ76 · A0A1S5WJ76_9REOV

  • Protein
    Non-structural protein 2
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Participates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments. Participates in the selective exclusion of host proteins from stress granules (SG) and P bodies (PB). Participates also in the sequestration of these remodeled organelles in viral factories.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, active site.

131720406080100120140160180200220240260280300
TypeIDPosition(s)Description
Binding site107-109ATP (UniProtKB | ChEBI)
Binding site188ATP (UniProtKB | ChEBI)
Binding site221-223ATP (UniProtKB | ChEBI)
Active site225For NTPase and RTPase activities
Binding site227ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell cytoplasm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionnucleoside diphosphate kinase activity
Molecular Functionribonucleoside triphosphate phosphatase activity
Molecular FunctionRNA binding
Biological Processviral genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Non-structural protein 2
  • EC number
  • Short names
    NSP2
  • Alternative names
    • NCVP3
    • Non-structural RNA-binding protein 35
      (NS35
      )

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • RVA/Pig-wt/TWN/1-4/2015/G9P19
    • RVA/Pig-wt/TWN/2-1/2015/G9P19
    • RVA/Pig-wt/TWN/3-20/2015/G9P19
    • RVA/Pig-wt/TWN/4-1/2015/G9P19
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Duplornaviricota > Resentoviricetes > Reovirales > Sedoreoviridae > Rotavirus > Rotavirus A

Accessions

  • Primary accession
    A0A1S5WJ76

Subcellular Location

Host cytoplasm
Note: Found in spherical cytoplasmic structures, called viral factories, that appear early after infection and are the site of viral replication and packaging.

Keywords

Interaction

Subunit

Homooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 phosphorylation and formation of viral factories. Interacts with host DCP1A, DCP1B, DDX6, EDC4 and EIF2S1/eIF2-alpha; these interactions are probably part of the sequestration of some host SGs and PBs proteins in viral factories.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain2-143Rotavirus non-structural protein 35 N-terminal
Domain144-316Rotavirus non-structural protein 35 C-terminal
Region205-241RNA-binding

Sequence similarities

Belongs to the rotavirus NSP2 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    317
  • Mass (Da)
    36,714
  • Last updated
    2017-05-10 v1
  • Checksum
    C1861ECD591D9E11
MAELACFCYPHLENDSYKFIPFNNLAIKCMLTAKVDKKDQDKFYNSIVYGIAPPPQFRKRYNTNDNSRGMNYETPMFNKVAMLICEALNSIKVTQSDVANVLSRVVSVRHLENLVLRKENHQDVLFHSKELLLKSVLIAIGQSKEIETTATAEGGEIVFQNTAFTMWKLTYLEHKLMPILDQNFIEYKITLNEDKPISDMCVKELVSELRWQYNRFAVITHGKGHYRVVKYSSVANHADRVFATYKNNVKSGNVTDFNLLDQRIIWQNWYAFTSSMKQGNTLDVCKKLLFQKMKHEKNPFKGLSTDRKMDEVSHVGI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KU739908
EMBL· GenBank· DDBJ
AQQ12231.1
EMBL· GenBank· DDBJ
Genomic RNA
KU739913
EMBL· GenBank· DDBJ
AQQ12234.1
EMBL· GenBank· DDBJ
Genomic RNA
KU739953
EMBL· GenBank· DDBJ
AQQ12273.1
EMBL· GenBank· DDBJ
Genomic RNA
KU739963
EMBL· GenBank· DDBJ
AQQ12283.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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